TY - JOUR
T1 - On the membrane binding of the potentiometric probe DI-4-anepps
T2 - A fluorescence and resonance raman study
AU - Pevzner, Eliyahu
AU - Ehrenberg, Benjamin
AU - Loew, Leslie M.
PY - 1993/8/1
Y1 - 1993/8/1
N2 - Di-4-ANEPPS is a dye molecules which has been used to probe membrane electric potentials by its electrochromic response. The dye has an unusual blue shift in the absorption spectrum upon partitioning to lipid membranes. We studied its binding properties to membranes by absorption, fluorescence and resonance Raman spectroscopy. The latter technique provided evidence for the differential solubilization of the dye in the membrane, where the polar head of the molecule protrudes out of the lipid environment and is responsible for the spectral behavior.
AB - Di-4-ANEPPS is a dye molecules which has been used to probe membrane electric potentials by its electrochromic response. The dye has an unusual blue shift in the absorption spectrum upon partitioning to lipid membranes. We studied its binding properties to membranes by absorption, fluorescence and resonance Raman spectroscopy. The latter technique provided evidence for the differential solubilization of the dye in the membrane, where the polar head of the molecule protrudes out of the lipid environment and is responsible for the spectral behavior.
KW - biological membranes
KW - potentiometric probe
KW - probe-membrane interaction
KW - resonance Raman spectroscopy
KW - styryl dye
UR - http://www.scopus.com/inward/record.url?scp=0007400524&partnerID=8YFLogxK
U2 - 10.1080/00387019308011602
DO - 10.1080/00387019308011602
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AN - SCOPUS:0007400524
SN - 0038-7010
VL - 26
SP - 1181
EP - 1193
JO - Spectroscopy Letters
JF - Spectroscopy Letters
IS - 7
ER -