On line monitoring of individual cells enzyme kinetic

Merav Sunray; Yana Shafran; Naomi Zurgil; Mordechai Deutsch

On line monitoring of individual cells enzyme kinetic

Merav Sunray, Yana Shafran, Naomi Zurgil, Mordechai Deutsch

Bar-Ilan University

Research output: Contribution to conference › Paper › peer-review

Abstract

A novel methodology for the measurement and analysis of apparent K_M (Michaelis-Menten constant) and V_MAX values of individual cells was established. Using microsive component, the time dependency of the Fluorescence Intensity (FI(t)) emitted from pre localized and defined fluorescein-diacetate (FDA) stained cells was recorded. Model simulations correlated with experimental results. Differences in distributions of individual K_M and V_MAX values of cells incubated with and without Phytohaemagglutinin (PHA) were evident. Average K_M and V_MAX of PHA stimulated cells increased by 99% and 540% correspondingly.

Original language	English
Pages	259
Number of pages	1
State	Published - 2001
Event	2001 International Semiconductor Conference - Siaia, Romania Duration: 9 Oct 2002 → 13 Oct 2002

Conference

Conference	2001 International Semiconductor Conference
Country/Territory	Romania
City	Siaia
Period	9/10/02 → 13/10/02

Cite this

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abstract = "A novel methodology for the measurement and analysis of apparent KM (Michaelis-Menten constant) and VMAX values of individual cells was established. Using microsive component, the time dependency of the Fluorescence Intensity (FI(t)) emitted from pre localized and defined fluorescein-diacetate (FDA) stained cells was recorded. Model simulations correlated with experimental results. Differences in distributions of individual KM and VMAX values of cells incubated with and without Phytohaemagglutinin (PHA) were evident. Average KM and VMAX of PHA stimulated cells increased by 99\% and 540\% correspondingly.",

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T1 - On line monitoring of individual cells enzyme kinetic

AU - Sunray, Merav

AU - Shafran, Yana

AU - Zurgil, Naomi

AU - Deutsch, Mordechai

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N2 - A novel methodology for the measurement and analysis of apparent KM (Michaelis-Menten constant) and VMAX values of individual cells was established. Using microsive component, the time dependency of the Fluorescence Intensity (FI(t)) emitted from pre localized and defined fluorescein-diacetate (FDA) stained cells was recorded. Model simulations correlated with experimental results. Differences in distributions of individual KM and VMAX values of cells incubated with and without Phytohaemagglutinin (PHA) were evident. Average KM and VMAX of PHA stimulated cells increased by 99% and 540% correspondingly.

AB - A novel methodology for the measurement and analysis of apparent KM (Michaelis-Menten constant) and VMAX values of individual cells was established. Using microsive component, the time dependency of the Fluorescence Intensity (FI(t)) emitted from pre localized and defined fluorescein-diacetate (FDA) stained cells was recorded. Model simulations correlated with experimental results. Differences in distributions of individual KM and VMAX values of cells incubated with and without Phytohaemagglutinin (PHA) were evident. Average KM and VMAX of PHA stimulated cells increased by 99% and 540% correspondingly.

UR - https://www.scopus.com/pages/publications/0035744278

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T2 - 2001 International Semiconductor Conference

Y2 - 9 October 2002 through 13 October 2002

ER -

On line monitoring of individual cells enzyme kinetic

Abstract

Conference

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