On binding of DNA-bending proteins to DNA minicircles

Shlomi Medalion, Yitzhak Rabin

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2 Scopus citations


We present a theoretical study of binding of DNA-bending proteins to circular DNA, using computer simulations of the wormlike chain model of DNA. We find that the binding affinity is affected by the bending elasticity and the conformational entropy of the polymer and that while protein adsorption is identical on open and closed long DNA molecules, there is significant enhancement of binding on DNA minicircles, compared to their linear counterparts. We also find that the ratio of the radii of gyration of open and closed chains depends on protein concentration for short DNA molecules. Experimental tests of our predictions are proposed.

Original languageEnglish
Article number025102
Pages (from-to)025102
JournalJournal of Chemical Physics
Issue number2
StatePublished - 14 Jan 2012

Bibliographical note

Funding Information:
This work was supported by grants from the Israel Science Foundation and from the US-Israel Binational Science Foundation. Helpful discussions with Jon Widom, John Marko, and David Bensimon are gratefully acknowledged.


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