TY - JOUR
T1 - Oligonucleotides are potent antioxidants acting mainly as metal-ion chelators
AU - Fischer, Bilha
AU - Zobel, Eyal
PY - 2008
Y1 - 2008
N2 - We explored by ESR the potential of 2'-deoxy-oligonucleotides as biocompatible inhibitors of the Fe(II)/Cu(I)/(II)-induced *OH formation from H(2)O(2). d(A)(5), (2'-OMe-A)(5), d(A)(7), d(A)(20), and d(T)(20), proved highly potent antioxidants (IC(50): 5-17 or 48-85 microM in inhibiting Fe(II)/Cu(I)- or Cu(II)-induced H(2)O(2)- decomposition), representing 40 to 215 - fold increase of potency as compared to Trolox. The antioxidant activity does not depend on the oligonucleotides' length or composition. The primary inhibition mechanism by oligonucleotides is metal-ion chelation and the secondary is radical scavenging. (1)H-, (31)P-NMR and ESR data suggest that Cu coordination involves adenine bases and 1-2 phosphates. We propose the use of short, metabolically stable oligonucleotides as highly potent and long-lived (t(1/2) ca. 20 h) antioxidants that may prevent oxidative damage.
AB - We explored by ESR the potential of 2'-deoxy-oligonucleotides as biocompatible inhibitors of the Fe(II)/Cu(I)/(II)-induced *OH formation from H(2)O(2). d(A)(5), (2'-OMe-A)(5), d(A)(7), d(A)(20), and d(T)(20), proved highly potent antioxidants (IC(50): 5-17 or 48-85 microM in inhibiting Fe(II)/Cu(I)- or Cu(II)-induced H(2)O(2)- decomposition), representing 40 to 215 - fold increase of potency as compared to Trolox. The antioxidant activity does not depend on the oligonucleotides' length or composition. The primary inhibition mechanism by oligonucleotides is metal-ion chelation and the secondary is radical scavenging. (1)H-, (31)P-NMR and ESR data suggest that Cu coordination involves adenine bases and 1-2 phosphates. We propose the use of short, metabolically stable oligonucleotides as highly potent and long-lived (t(1/2) ca. 20 h) antioxidants that may prevent oxidative damage.
UR - http://www.scopus.com/inward/record.url?scp=78649410013&partnerID=8YFLogxK
U2 - 10.1093/nass/nrn246
DO - 10.1093/nass/nrn246
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C2 - 18776465
AN - SCOPUS:78649410013
SN - 1746-8272
SP - 485
EP - 486
JO - Nucleic acids symposium series (2004)
JF - Nucleic acids symposium series (2004)
IS - 52
ER -