Old questions, new tools: Does next-generation sequencing hold the key to unraveling intestinal B-cell responses?

O. Pabst; H. Hazanov; R. Mehr

doi:10.1038/mi.2014.103

Old questions, new tools: Does next-generation sequencing hold the key to unraveling intestinal B-cell responses?

O. Pabst, H. Hazanov, R. Mehr

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

RWTH Aachen University

Research output: Contribution to journal › Review article › peer-review

9 Scopus citations

Abstract

Analysis of the intestinal B-cell system and properties of immunoglobulin A, the main antibody isotype produced in the gut, has dominated the rise of mucosal immunology as a discipline. Seminal work established concepts describing the induction, transport, and function of mucosal antibodies. Still, open questions remain and we lack a comprehensive view of how the various sites and pathways of immunoglobulin A induction are integrated to respond to gut antigens. Next-generation sequencing (NGS) offers a novel approach to study B-cell responses, which might substantially enhance our tool box to answer key questions in the field and to take the next steps toward therapeutic exploitation of the mucosal B-cell system. In this review we discuss the potential, challenges, and emerging solutions for gut B-cell repertoire analysis by NGS.

Original language	English
Pages (from-to)	29-37
Number of pages	9
Journal	Mucosal Immunology
Volume	8
Issue number	1
Early online date	12 Nov 2014
DOIs	https://doi.org/10.1038/mi.2014.103
State	Published - 11 Jan 2015

Bibliographical note

Funding Information:
We are indebted to Miri Michaeli, Irina Iosselevitch, and Olga Schulz for help in figure and manuscript preparation. This work was supported by Deutsche Forschungsgemeinschaft grant PA921/4-1 to O.P. and Israel Science Foundation grant 270/09, a Human Frontiers Science Program Research Grant and a grant from the Bar Ilan University–Sheba medical Center joint research fund to R.M.

Funding

We are indebted to Miri Michaeli, Irina Iosselevitch, and Olga Schulz for help in figure and manuscript preparation. This work was supported by Deutsche Forschungsgemeinschaft grant PA921/4-1 to O.P. and Israel Science Foundation grant 270/09, a Human Frontiers Science Program Research Grant and a grant from the Bar Ilan University–Sheba medical Center joint research fund to R.M.

Funders	Funder number
Bar Ilan University–Sheba medical Center
Deutsche Forschungsgemeinschaft	PA921/4-1
Israel Science Foundation	270/09

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Old questions, new tools: Does next-generation sequencing hold the key to unraveling intestinal B-cell responses?

Abstract

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