odd Oz: A novel Drosophila pair rule gene

Anna Levine, Ayelet Bashan-Ahrend, Ofra Budai-Hadrian, Devorah Gartenberg, Sophia Menasherow, Ron Wides

    Research output: Contribution to journalArticlepeer-review

    131 Scopus citations

    Abstract

    We have identified a novel pair rule gene in Drosophila, odd Oz (odz). Every odd-numbered body segment is deleted in odz mutant embryos. The odz gene product is strongly expressed in the embryonic central nervous system and heart, and both of these tissues are malformed in mutant embryos. odz represents the only known pair rule gene that does not encode a transcription factor. Instead, it encodes a protein with EGF-like repeats homologous to those of the extracellular matrix protein tenascin. The protein is also a putative transmembrane tyrosine kinase substrate. On the basis of its structure, odz must act in a cellularized embryo. This is consistent with odz expression, whose temporal appearance is indicative of a very late-acting pair rule gene.

    Original languageEnglish
    Pages (from-to)587-598
    Number of pages12
    JournalCell
    Volume77
    Issue number4
    DOIs
    StatePublished - 20 May 1994

    Bibliographical note

    Funding Information:
    Address correspondence to R. W. We thank Lilly Glazer for initial localization of the DNA to 79E, Orit Leitner for work on production of monoclonal antibody, Tevie Mehlman for sequencing expertise, Chaim Prilusky for assistance with DNA sequencing programs, Tami Eiger and Sylvia Markovitz for photography, and Talila Volk for use of her antibody against pericardioblasts before publication. For helpful discussions, we thank Benny Shilo and the members of his lab, Christian Kliimbt, Eric Weischaus, Talila Volk, Ron Goldstein, and Erez Raz. Thanks to Don Katcoff, Benny Shilo, and Talila Volk for critical reading of this manuscript. R. W. is an Israel Cancer Research Fund fellow and Israel Cancer Association fellow. R. W. and A. L. have both received support from the Israel Ministry of Absorption. Portions of this project have been supported by the Israel Ministries of Health and of Science and Technology. Drosophila stocks were kindly provided by the Bloomington and Bowling Green stock centers, Eric Weischaus, Allan Spradling, James Kennison, Stanley Tiong, Christiane Niisslein-Volhard, Talila Volk, and Benny Shilo.

    Funding

    Address correspondence to R. W. We thank Lilly Glazer for initial localization of the DNA to 79E, Orit Leitner for work on production of monoclonal antibody, Tevie Mehlman for sequencing expertise, Chaim Prilusky for assistance with DNA sequencing programs, Tami Eiger and Sylvia Markovitz for photography, and Talila Volk for use of her antibody against pericardioblasts before publication. For helpful discussions, we thank Benny Shilo and the members of his lab, Christian Kliimbt, Eric Weischaus, Talila Volk, Ron Goldstein, and Erez Raz. Thanks to Don Katcoff, Benny Shilo, and Talila Volk for critical reading of this manuscript. R. W. is an Israel Cancer Research Fund fellow and Israel Cancer Association fellow. R. W. and A. L. have both received support from the Israel Ministry of Absorption. Portions of this project have been supported by the Israel Ministries of Health and of Science and Technology. Drosophila stocks were kindly provided by the Bloomington and Bowling Green stock centers, Eric Weischaus, Allan Spradling, James Kennison, Stanley Tiong, Christiane Niisslein-Volhard, Talila Volk, and Benny Shilo.

    FundersFunder number
    Israel Ministries of Health and of Science and Technology

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