Ocular inflammation stimulated by the immunomodulator AS101 [ammonium trichloro(dioxyethelene-o-o') tellurate]

Kourosh Dastgheib, Naofumi Hikita, Benjamin Sredni, Michael Albeck, Devora Sredni, Robert B. Nussenblatt, Chi Chao Chan

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2 Scopus citations

Abstract

The purpose of this study was to investigate the effect of a novel immunomodulator, AS101 [ammonium trichloro(dioxyethelene-O-O') tellurate], in the eye. Lewis rats were injected intravitreally with AS 101 at a concentration of 13 μg/ml in one eye and BSS in the contralateral eye. Control animals were injected with BSS into the central vitreous of both eyes. Ocular inflammation was evaluated at 20 hours by histology, immunopathology, and by cell count, protein and cytokine measurement in the aqueous humor. At 20 hours, eyes injected with AS101 developed iridocyclitis and mild vitritis versus minimal inflammation and/or protein in contralateral eyes or eyes of control animals (p = 0.0121). The inflammatory infiltrate was mixed in character. Major Histocompatibility Complex (MHC) class II antigens and intercellular adhesion molecules (ICAM-1) were expressed in the anterior segment of eyes injected with AS101. In the aqueous humor of these eyes there were significant quantities of inflammatory cells, protein (mean ± SEM = 11.2 ± 2.3 mg/ml) and the cytokine interleukin 6 (IL-6) (450 units/ml) compared with contralateral eyes (p = 0.0005 for inflammatory cells; protein, mean ± SEM = 1.6 ± 0.17 mg/ml; IL-6 = 12 units/ml) and both eyes of control animals injected with BSS (p = 0.8955 for inflammatory cells; protein, OD = 1.5 mg/ml, OS = 0.7 mg/ml; IL-6, OD = 8 units/ml, OS = 13 units/ml). AS101 has a local inflammatory effect in the eye. This compound may activate ocular inflammation by releasing cytokines such as IL-6.

Original languageEnglish
Pages (from-to)603-610
Number of pages8
JournalCurrent Eye Research
Volume13
Issue number8
DOIs
StatePublished - 1994

Bibliographical note

Funding Information:
This study was supported in part by the Milton and Lois Shiffman NIH-C.A.I.R. Institute/Bar nan University Research Program, Ramat Gan, Israel.

Keywords

  • AS101
  • Immunomodulator
  • Interleukin-6
  • Intravitreal injection
  • Ocular inflammation
  • Rat

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