Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells

Marilena Ciciarello, Emanuele Roscioli, Barbara Di Fiore, Laura Di Francesco, Fabrizia Sobrero, Delphine Bernard, Rosamaria Mangiacasale, Amnon Harel, Maria Eugenia Schininà, Patrizia Lavia

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20 Scopus citations

Abstract

The GTPase Ran regulates nucleocytoplasmic transport in interphase and spindle organisation in mitosis via effectors of the importin beta superfamily. Ran-binding protein 1 (RanBP1) regulates guanine nucleotide turnover on Ran, as well as its interactions with effectors. Unlike other Ran network members that are steadily expressed, RanBP1 abundance is modulated during the mammalian cell cycle, peaking in mitosis and declining at mitotic exit. Here, we show that RanBP1 downregulation takes place in mid to late telophase, concomitant with the reformation of nuclei. Mild RanBP1 overexpression in murine cells causes RanBP1 to persist in late mitosis and hinders a set of events underlying the telophase to interphase transition, including chromatin decondensation, nuclear expansion and nuclear lamina reorganisation. Moreover, the reorganisation of nuclear pores fails associated with defective nuclear relocalisation of NLS cargoes. Co-expression of importin beta, together with RanBP1, however mitigates these defects. Thus, RanBP1 downregulation is required for nuclear reorganisation pathways operated by importin beta after mitosis.

Original languageEnglish
Pages (from-to)651-668
Number of pages18
JournalChromosoma
Volume119
Issue number6
DOIs
StatePublished - Dec 2010
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgements We are extremely grateful to Antonio Tedeschi (IMP, Vienna) for contributing to early stages of this work and to Giulia Guarguaglini (IBPM, Rome) for insightful comments on this manuscript. This work was supported by MIUR - Italian Ministry of University and Research (FIRB grant RBIN04T7MT and PRIN grant 200879X9N9-004), Associazione Italiana per la Ricerca sul Cancro (AIRC) and by funds from Assicurazioni Generali and Fondazione Roma Terzo Settore.

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