TY - JOUR
T1 - Novel trypanosomatid small nucleolar RNAs that guide methylation: Their genome organization, expression and potential use to direct specific methylation on target RNA molecules
T2 - Their genome organization, expression and potential use to direct specific methylation on target RNA molecules
AU - Xu, Y. X.
AU - Liu, L.
AU - Michaeli, S.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000/8/22
Y1 - 2000/8/22
N2 - Trypanosomatids are the causative agent of several major parasitic diseases including African trypanosomiasis, American trypanosomiasis, and leishmaniasis. These parasites possess unique RNA-processing mechanisms including trans-splicing of pre-mRNA and RNA editing of mitochondrial transcripts. In this study, we identified a trypanosomatid novel group of small nucleolar RNAs that belong to the box C/D snoRNA, which were shown to guide ribose methylation on rRNA. Three snoRNA genes were identified; snoRNA-2 carrying a single snoRNA and g2 and b2 coding for a single or multiple snoRNAs, respectively. Mapping of the methylation sites guided by snoRNA-2 using two different approaches suggest that snoRNA-2 has the potential to guide methylation on both 5.8S anb 18S rRNAs. The trypanosomes follow the same guide-methylation rule established for yeast and for mammals. As a first attempt to change the methylation pattern of target RNAs, we generated transgenic parasites carrying the B2 and snoRNA-2, which were engineered to shift the methylation site on rRNA. Despite efficient expression of these tagged snoRNAs, the novel methylation site was not generated. However, efficient expression of tagged snoRNAs in transgenic parasites opens the possibility of engineering novel methylation sites on different target RNAs in vivo.
AB - Trypanosomatids are the causative agent of several major parasitic diseases including African trypanosomiasis, American trypanosomiasis, and leishmaniasis. These parasites possess unique RNA-processing mechanisms including trans-splicing of pre-mRNA and RNA editing of mitochondrial transcripts. In this study, we identified a trypanosomatid novel group of small nucleolar RNAs that belong to the box C/D snoRNA, which were shown to guide ribose methylation on rRNA. Three snoRNA genes were identified; snoRNA-2 carrying a single snoRNA and g2 and b2 coding for a single or multiple snoRNAs, respectively. Mapping of the methylation sites guided by snoRNA-2 using two different approaches suggest that snoRNA-2 has the potential to guide methylation on both 5.8S anb 18S rRNAs. The trypanosomes follow the same guide-methylation rule established for yeast and for mammals. As a first attempt to change the methylation pattern of target RNAs, we generated transgenic parasites carrying the B2 and snoRNA-2, which were engineered to shift the methylation site on rRNA. Despite efficient expression of these tagged snoRNAs, the novel methylation site was not generated. However, efficient expression of tagged snoRNAs in transgenic parasites opens the possibility of engineering novel methylation sites on different target RNAs in vivo.
KW - Parasitic diseases
KW - Small nucleolar RNA
KW - Trypanosome
UR - http://www.scopus.com/inward/record.url?scp=33865928&partnerID=8YFLogxK
UR - https://scholar.google.co.il/scholar?q=Novel+trypanosomatid+small+nucleolar+RNAs+that+guide+methylation%3A+their+genome+organization%2C+expression+and+potential+use+to+direct+specific+methylation+on+target+RNA+molecules&btnG=&hl=en&as_sdt=0%2C5
UR - http://www.scopus.com/inward/record.url?scp=0033865928&partnerID=8YFLogxK
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C2 - 10909419
SN - 1565-1088
VL - 2
SP - 58
EP - 62
JO - Israel Medical Association Journal
JF - Israel Medical Association Journal
IS - SUPPL. JULY
ER -