TY - JOUR
T1 - Novel effective immunoadsorbents based on agarose-polyaldehyde microsphere beads
T2 - Synthesis and affinity chromatography
AU - Margel, S.
AU - Offarim, M.
PY - 1983/2/1
Y1 - 1983/2/1
N2 - Agarose-polyaldehyde microsphere beads were produced by encapsulating polyacrolein microspheres or polyglutaraldehyde microspheres with agarose. Magnetic beads were formed by carrying out the encapsulation procedure in the presence of ferrofluidic particles. Proteins were bound covalently, at physiological pH, to the beads through their aldehyde groups to produce the Schiff base products. The conjugates, beads-proteins, were used successfully in affinity chromatography for specific purification of antibodies. Leaching of proteins bound to the beads under physiological conditions and eluting conditions was not detected. The agarose-polyaldehyde microsphere beads are suggested as alternatives to the supports currently used in affinity chromatography.
AB - Agarose-polyaldehyde microsphere beads were produced by encapsulating polyacrolein microspheres or polyglutaraldehyde microspheres with agarose. Magnetic beads were formed by carrying out the encapsulation procedure in the presence of ferrofluidic particles. Proteins were bound covalently, at physiological pH, to the beads through their aldehyde groups to produce the Schiff base products. The conjugates, beads-proteins, were used successfully in affinity chromatography for specific purification of antibodies. Leaching of proteins bound to the beads under physiological conditions and eluting conditions was not detected. The agarose-polyaldehyde microsphere beads are suggested as alternatives to the supports currently used in affinity chromatography.
UR - http://www.scopus.com/inward/record.url?scp=0020711001&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(83)90384-6
DO - 10.1016/0003-2697(83)90384-6
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C2 - 6846811
AN - SCOPUS:0020711001
SN - 0003-2697
VL - 128
SP - 342
EP - 350
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -