TY - JOUR
T1 - Novel biocompatible hydrogel nanoparticles
T2 - generation and size-tuning of nanoparticles by the formation of micelle templates obtained from thermo-responsive monomers mixtures
AU - Khandadash, Raz
AU - Machtey, Victoria
AU - Shainer, Inbal
AU - Gottlieb, Hugo E.
AU - Gothilf, Yoav
AU - Ebenstein, Yuval
AU - Weiss, Aryeh
AU - Byk, Gerardo
N1 - Publisher Copyright:
© 2014, Springer Science+Business Media Dordrecht.
PY - 2014/12
Y1 - 2014/12
N2 - Biocompatible hydrogel nanoparticles are prepared by polymerization and cross-linking of N-isopropyl acrylamide in a micelle template formed by block copolymers macro-monomers at high temperature. Different monomer ratios form, at high temperature, well-defined micelles of different sizes which are further polymerized leading to nanoparticles with varied sizes from 20 to 390 nm. Physico-chemical characterization of the nanoparticles demonstrates their composition and homogeneity. The NPs were tested in vitro and in vivo biocompatibility assays, and their lack of toxicity was proven. The NPs can be labeled with fluorescent probes, and their intracellular fate can be visualized and quantified using confocal microscopy. Their uptake by live stem cells and distribution in whole developing animals is reported. On the basis of our results, a mechanism of nanoparticle formation is suggested. The lack of toxicity makes these nanoparticles especially attractive for biological applications such as screening and bio-sensing.
AB - Biocompatible hydrogel nanoparticles are prepared by polymerization and cross-linking of N-isopropyl acrylamide in a micelle template formed by block copolymers macro-monomers at high temperature. Different monomer ratios form, at high temperature, well-defined micelles of different sizes which are further polymerized leading to nanoparticles with varied sizes from 20 to 390 nm. Physico-chemical characterization of the nanoparticles demonstrates their composition and homogeneity. The NPs were tested in vitro and in vivo biocompatibility assays, and their lack of toxicity was proven. The NPs can be labeled with fluorescent probes, and their intracellular fate can be visualized and quantified using confocal microscopy. Their uptake by live stem cells and distribution in whole developing animals is reported. On the basis of our results, a mechanism of nanoparticle formation is suggested. The lack of toxicity makes these nanoparticles especially attractive for biological applications such as screening and bio-sensing.
KW - Cellular tracking
KW - N-isopropyl-acrylamide
KW - Polyethylene oxide
KW - Self-assembly
KW - Thermo-responsive material
KW - Toxicity of nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84917690792&partnerID=8YFLogxK
U2 - 10.1007/s11051-014-2796-1
DO - 10.1007/s11051-014-2796-1
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
SN - 1388-0764
VL - 16
JO - Journal of Nanoparticle Research
JF - Journal of Nanoparticle Research
IS - 12
M1 - 2796
ER -