TY - JOUR
T1 - Nontransformed, GM-CSF-dependent macrophage lines are a unique model to study tissue macrophage functions
AU - Fejer, György
AU - Wegner, Mareike Dorothee
AU - Györy, Ildiko
AU - Cohen, Idan
AU - Engelhard, Peggy
AU - Voronov, Elena
AU - Manke, Thomas
AU - Ruzsics, Zsolt
AU - Dölken, Lars
AU - Da Costa, Olivia Prazeres
AU - Branzk, Nora
AU - Huber, Michael
AU - Prasse, Antje
AU - Schneider, Robert
AU - Apte, Ron N.
AU - Galanos, Chris
AU - Freudenberg, Marina A.
PY - 2013/6/11
Y1 - 2013/6/11
N2 - Macrophages are diverse cell types in the first line of antimicrobial defense. Only a limited number of primary mouse models exist to study their function. Bone marrow-derived, macrophage-CSF-induced cells with a limited life span are the most common source. We report here a simple method yielding self-renewing, nontransformed, GM-CSF/signal transducer and activator of transcription 5-dependent macrophages (Max Planck Institute cells) from mouse fetal liver, which reflect the innate immune characteristics of alveolar macrophages. Max Planck Institute cells are exquisitely sensitive to selected microbial agents, including bacterial LPS, lipopeptide, Mycobacterium tuberculosis, cord factor, and adenovirus and mount highly proinflammatory but no anti-inflammatory IL-10 responses. They show a unique pattern of innate responses not yet observed in other mononuclear phagocytes. This includes differential LPS sensing and an unprecedented regulation of IL-1α production upon LPS exposure, which likely plays a key role in lung inflammation in vivo. In conclusion, Max Planck Institute cells offer an useful tool to study macrophage biology and for biomedical science.
AB - Macrophages are diverse cell types in the first line of antimicrobial defense. Only a limited number of primary mouse models exist to study their function. Bone marrow-derived, macrophage-CSF-induced cells with a limited life span are the most common source. We report here a simple method yielding self-renewing, nontransformed, GM-CSF/signal transducer and activator of transcription 5-dependent macrophages (Max Planck Institute cells) from mouse fetal liver, which reflect the innate immune characteristics of alveolar macrophages. Max Planck Institute cells are exquisitely sensitive to selected microbial agents, including bacterial LPS, lipopeptide, Mycobacterium tuberculosis, cord factor, and adenovirus and mount highly proinflammatory but no anti-inflammatory IL-10 responses. They show a unique pattern of innate responses not yet observed in other mononuclear phagocytes. This includes differential LPS sensing and an unprecedented regulation of IL-1α production upon LPS exposure, which likely plays a key role in lung inflammation in vivo. In conclusion, Max Planck Institute cells offer an useful tool to study macrophage biology and for biomedical science.
KW - Innate immunity
KW - LPS recognition
UR - http://www.scopus.com/inward/record.url?scp=84878960401&partnerID=8YFLogxK
U2 - 10.1073/pnas.1302877110
DO - 10.1073/pnas.1302877110
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 23708119
AN - SCOPUS:84878960401
SN - 0027-8424
VL - 110
SP - E2191-E2198
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 24
ER -
