Non-labeled lensless micro-endoscopic approach for cellular imaging through highly scattering media

Omer Wagner, Aditya Pandya, Yoav Chemla, Hadar Pinhas, Irina Schelkanova, Asaf Shahmoon, Yossi Mandel, Alexandre Douplik, Zeev Zalevsky

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


We describe an imaging approach based on an optical setup made up of a miniature, lensless, minimally invasive endoscope scanning a sample and matching post processing techniques that enable enhanced imaging capabilities. The two main scopes of this article are that this approach enables imaging beyond highly scattering medium and increases the resolution and signal to noise levels reaching single cell imaging. Our approach has more advantages over ordinary endoscope setups and other imaging techniques. It is not mechanically limited by a lens, the stable but flexible fiber can acquire images over long time periods (unlike current imaging methods such as OCT etc.), and the imaging can be obtained at a certain working distance above the surface, without interference to the imaged object. Fast overlapping scans enlarge the region of interest, enhance signal to noise levels and can also accommodate post-processing, super-resolution algorithms. Here we present that due to the setup properties, the overlapping scans also lead to dramatic enhancement of non-scattered signal to scattered noise. This enables imaging through highly scattering medium. We discuss results obtained from in vitro investigation of weak signals of ARPE cells, rat retina, and scattered signals from polydimethylsiloxane (PDMS) microchannels filled with hemoglobin and covered by intralipids consequently mimicking blood capillaries and the epidermis of human skin. The development of minimally invasive procedures and methodologies for imaging through scattering medium such as tissues can vastly enhance biomedical diagnostic capabilities for imaging internal organs. We thereby propose that our method may be used for such tasks in vivo.-c 2018 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).

Original languageEnglish
Article numberBSR20170027
JournalBioscience Reports
Issue number1
StatePublished - 25 Jan 2018

Bibliographical note

Funding Information:
This work was partially supported by the Israeli Ministry of Science, Technology & Space doctoral scholarship for Y.C.


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