TY - JOUR
T1 - No intraindividual variation of disomy rate in sperm samples
AU - Amiel, A.
AU - Bartoov, B.
AU - Pevsner, D.
AU - Sardos-Albertini, F.
AU - Fejgin, M. D.
PY - 2002
Y1 - 2002
N2 - We assessed possible inter- and intraindividual variations in the frequency of disomy in sperm cells from three men with abnormal sperm analysis parameters. Mono- and dual-color fluorescence in situ hybridization (FISH) was applied to sperm cells from different samples of the men. Four men with a normal sperm profile were used as controls. The samples were taken separately over a period of 6 months. FISH probes used for the disomy rate analysis were clones from the satellite region of chromosomes 8, 18, X, and Y. The study group showed a significantly higher disomy rate compared with the control group, whereas there was no significant difference in the disomy rate between three different samples from the same individuals. These results suggest that the sampling time has no importance in assessing the rate of nondisjunction in sperm cells.
AB - We assessed possible inter- and intraindividual variations in the frequency of disomy in sperm cells from three men with abnormal sperm analysis parameters. Mono- and dual-color fluorescence in situ hybridization (FISH) was applied to sperm cells from different samples of the men. Four men with a normal sperm profile were used as controls. The samples were taken separately over a period of 6 months. FISH probes used for the disomy rate analysis were clones from the satellite region of chromosomes 8, 18, X, and Y. The study group showed a significantly higher disomy rate compared with the control group, whereas there was no significant difference in the disomy rate between three different samples from the same individuals. These results suggest that the sampling time has no importance in assessing the rate of nondisjunction in sperm cells.
KW - Disomy rate
KW - Intraindividual variation
KW - Sperm cell
UR - http://www.scopus.com/inward/record.url?scp=0036032094&partnerID=8YFLogxK
U2 - 10.1007/s100380200081
DO - 10.1007/s100380200081
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AN - SCOPUS:0036032094
SN - 1434-5161
VL - 47
SP - 539
EP - 542
JO - Journal of Human Genetics
JF - Journal of Human Genetics
IS - 10
ER -