NMR-based molecular ruler for determining the depth of intercalants within the lipid bilayer. Part V: A comparison of liposomes, bioliposomes and erythrocyte ghosts

Michal Afri, Carmit Alexenberg, Efrat Bodner, Aryeh A. Frimer, Abraham I. Jacob, Miriam E. Naqqash

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Afri et al. (2014a,b) have recently reported their mapping of DMPC liposomes using 13C NMR in conjunction with a wide range of difunctional intercalants: n-ketoesters, n-ketoacids and n-ketophosphatidylcholines. The present study initiates a comparable study of bioliposomes and erythrocyte ghosts. This required the 13C NMR characterization of these systems for the first time, and further involved a determination of the signals of three doubly 13C-labeled intercalants, in particular, n-ketophosphatidylcholines where n=4, 8 and 12. This study reveals that DMPC liposomes, bioliposomes and erythrocyte ghosts, with all their structural differences, are not radically different from the perspective of polarity gradient. Any differences observed reflect the additives often naturally present in these lipid systems.

Original languageEnglish
Pages (from-to)52-60
Number of pages9
JournalChemistry and Physics of Lipids
Volume184
DOIs
StatePublished - Dec 2014

Bibliographical note

Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.

Funding

We acknowledge the kind and generous support of the Israel Science Foundation (Grant Numbers 327/02 and 437/06 ) – founded by The Israel Academy of Sciences and Humanities and The Ethel and David Resnick Chair in Active Oxygen Chemistry.

FundersFunder number
Israel Academy of Sciences and Humanities
Israel Science Foundation327/02, 437/06

    Keywords

    • Bioliposome
    • C NMR
    • DMPC liposome
    • E(30)
    • Erythrocyte ghost
    • Ketophospholipids

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