Afri et al. (2014a,b) have recently reported their mapping of DMPC liposomes using 13C NMR in conjunction with a wide range of difunctional intercalants: n-ketoesters, n-ketoacids and n-ketophosphatidylcholines. The present study initiates a comparable study of bioliposomes and erythrocyte ghosts. This required the 13C NMR characterization of these systems for the first time, and further involved a determination of the signals of three doubly 13C-labeled intercalants, in particular, n-ketophosphatidylcholines where n=4, 8 and 12. This study reveals that DMPC liposomes, bioliposomes and erythrocyte ghosts, with all their structural differences, are not radically different from the perspective of polarity gradient. Any differences observed reflect the additives often naturally present in these lipid systems.
Bibliographical noteFunding Information:
We acknowledge the kind and generous support of the Israel Science Foundation (Grant Numbers 327/02 and 437/06 ) – founded by The Israel Academy of Sciences and Humanities and The Ethel and David Resnick Chair in Active Oxygen Chemistry.
© 2014 Elsevier B.V. All rights reserved.
- C NMR
- DMPC liposome
- Erythrocyte ghost