NMR-based molecular ruler for determining the depth of intercalants within the lipid bilayer. Part II. The preparation of a molecular ruler

Yael Cohen; Michal Afri; Aryeh A. Frimer

doi:10.1016/j.chemphyslip.2008.07.007

NMR-based molecular ruler for determining the depth of intercalants within the lipid bilayer. Part II. The preparation of a molecular ruler

Yael Cohen, Michal Afri, Aryeh A. Frimer

Department of Chemistry

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We have previously shown how the location of an intercalant within the lipid bilayer can be qualitatively determined by using the excellent correlation that exists between the ¹³C NMR chemical shift of a polarizable carbon (e.g., the carbonyl or nitronyl carbon) and the polarity (using the Dimroth-Reichardt's E_T(30) parameter) of the microenvironment in which that carbon resides. In a companion paper, we have determined criteria for reporter molecules that will assist us in converting this qualitative polarity data into quantitative Angstrom values. In the present paper, we report on our initial success in quantitatively mapping of the DMPC bilayer by linking two or more vertical points within a bilayer by both distance (in Angstroms) and E_T(30) polarity. The results correlated well with the values obtained using the "parallax method" of Erwin London.

Original language	English
Pages (from-to)	114-119
Number of pages	6
Journal	Chemistry and Physics of Lipids
Volume	155
Issue number	2
DOIs	https://doi.org/10.1016/j.chemphyslip.2008.07.007
State	Published - Oct 2008

Bibliographical note

Funding Information:
We acknowledge the kind and generous support of the Israel Science Foundation (Grants Number 327/02 and 437/06) – founded by The Israel Academy of Sciences and Humanities, and The Ethel and David Resnick Chair in Active Oxygen Chemistry.

Funding

We acknowledge the kind and generous support of the Israel Science Foundation (Grants Number 327/02 and 437/06) – founded by The Israel Academy of Sciences and Humanities, and The Ethel and David Resnick Chair in Active Oxygen Chemistry.

Funders	Funder number
Israel Academy of Sciences and Humanities
Israel Science Foundation	327/02, 437/06

Keywords

C NMR
DMPC
E(30)
Liposome
Membrane
Molecular ruler
Parallax method

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10.1016/j.chemphyslip.2008.07.007

Cite this

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abstract = "We have previously shown how the location of an intercalant within the lipid bilayer can be qualitatively determined by using the excellent correlation that exists between the 13C NMR chemical shift of a polarizable carbon (e.g., the carbonyl or nitronyl carbon) and the polarity (using the Dimroth-Reichardt's ET(30) parameter) of the microenvironment in which that carbon resides. In a companion paper, we have determined criteria for reporter molecules that will assist us in converting this qualitative polarity data into quantitative Angstrom values. In the present paper, we report on our initial success in quantitatively mapping of the DMPC bilayer by linking two or more vertical points within a bilayer by both distance (in Angstroms) and ET(30) polarity. The results correlated well with the values obtained using the {"}parallax method{"} of Erwin London.",

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note = "Funding Information: We acknowledge the kind and generous support of the Israel Science Foundation (Grants Number 327/02 and 437/06) – founded by The Israel Academy of Sciences and Humanities, and The Ethel and David Resnick Chair in Active Oxygen Chemistry.",

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AU - Afri, Michal

AU - Frimer, Aryeh A.

N1 - Funding Information: We acknowledge the kind and generous support of the Israel Science Foundation (Grants Number 327/02 and 437/06) – founded by The Israel Academy of Sciences and Humanities, and The Ethel and David Resnick Chair in Active Oxygen Chemistry.

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N2 - We have previously shown how the location of an intercalant within the lipid bilayer can be qualitatively determined by using the excellent correlation that exists between the 13C NMR chemical shift of a polarizable carbon (e.g., the carbonyl or nitronyl carbon) and the polarity (using the Dimroth-Reichardt's ET(30) parameter) of the microenvironment in which that carbon resides. In a companion paper, we have determined criteria for reporter molecules that will assist us in converting this qualitative polarity data into quantitative Angstrom values. In the present paper, we report on our initial success in quantitatively mapping of the DMPC bilayer by linking two or more vertical points within a bilayer by both distance (in Angstroms) and ET(30) polarity. The results correlated well with the values obtained using the "parallax method" of Erwin London.

AB - We have previously shown how the location of an intercalant within the lipid bilayer can be qualitatively determined by using the excellent correlation that exists between the 13C NMR chemical shift of a polarizable carbon (e.g., the carbonyl or nitronyl carbon) and the polarity (using the Dimroth-Reichardt's ET(30) parameter) of the microenvironment in which that carbon resides. In a companion paper, we have determined criteria for reporter molecules that will assist us in converting this qualitative polarity data into quantitative Angstrom values. In the present paper, we report on our initial success in quantitatively mapping of the DMPC bilayer by linking two or more vertical points within a bilayer by both distance (in Angstroms) and ET(30) polarity. The results correlated well with the values obtained using the "parallax method" of Erwin London.

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KW - E(30)

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NMR-based molecular ruler for determining the depth of intercalants within the lipid bilayer. Part II. The preparation of a molecular ruler

Abstract

Bibliographical note

Funding

Keywords

Access to Document

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