Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

Qing Dai; Sharon Moshitch-Moshkovitz; Dali Han; Nitzan Kol; Ninette Amariglio; Gideon Rechavi; Dan Dominissini; Chuan He

doi:10.1038/nmeth.4294

Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

Qing Dai
, Sharon Moshitch-Moshkovitz
, Dali Han
, Nitzan Kol
, Ninette Amariglio
, Gideon Rechavi
, Dan Dominissini
, Chuan He

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

The University of Chicago
Sheba Medical Center at Tel Hashomer
Tel Aviv University

Research output: Contribution to journal › Article › peer-review

252 Scopus citations

Abstract

The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

Original language	English
Pages (from-to)	695-698
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	7
Early online date	15 May 2017
DOIs	https://doi.org/10.1038/nmeth.4294
State	Published - Jul 2017

Bibliographical note

Publisher Copyright:
© 2017 Nature America, Inc., part of Springer Nature. All rights reserved.

Funding

This work was supported by the US National Institutes of Health NHGRI RM1 HG008935 to C.H.; a grant from the Kahn Family Foundation to D.D. and G.R.; and grants from the Ernest and Bonnie Beutler Research Program, Flight Attendant Medical Research Institute (FAMRI) and the Israeli Centers of Excellence (I-CORE) Program (ISF grants no. 41/11 and no. 1796/12) to G.R. C.H. is an investigator of the Howard Hughes Medical Institute (HHMI). G.R. is a member of the Sagol Neuroscience Network and holds the Djerassi Chair for Oncology at the Sackler Faculty of Medicine, Tel-Aviv University, Israel. D.D. was supported by a Human Frontier Science Program (HFSP) long-term fellowship. Q.D. is supported by the National Institutes of Health grant K01 HG006699. We wish to thank M. Salmon-Divon for advice and help with bioinformatic analysis and R. Mashiach for help with chemical structure drawings.

Funders	Funder number
National Institutes of Health	NHGRI RM1 HG008935, K01 HG006699
Howard Hughes Medical Institute
National Cancer Institute	U54CA193419
Esther B. Kahn Charitable Foundation
Flight Attendant Medical Research Institute
University of Miami
Assam University, Silchar
Human Frontier Science Program
Israel Science Foundation	41/11, 1796/12
Japanese Brain Bank Network for Neuroscience Research
Israeli Centers for Research Excellence

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Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

Abstract

Bibliographical note

Funding

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