Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

Qing Dai; Sharon Moshitch-Moshkovitz; Dali Han; Nitzan Kol; Ninette Amariglio; Gideon Rechavi; Dan Dominissini; Chuan He

doi:10.1038/nmeth.4294

Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

Qing Dai, Sharon Moshitch-Moshkovitz, Dali Han, Nitzan Kol, Ninette Amariglio, Gideon Rechavi, Dan Dominissini, Chuan He

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

213 Scopus citations

Abstract

The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

Original language	English
Pages (from-to)	695-698
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	7
Early online date	15 May 2017
DOIs	https://doi.org/10.1038/nmeth.4294
State	Published - Jul 2017

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abstract = "The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.",

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AU - Dominissini, Dan

AU - He, Chuan

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Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

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