NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera

Xavier Michalet; Arin C. Ulku; Michael A. Wayne; Shimon Weiss; Claudio Bruschini; Edoardo Charbon

doi:10.1117/12.2649227

NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera

Xavier Michalet, Arin C. Ulku, Michael A. Wayne, Shimon Weiss, Claudio Bruschini, Edoardo Charbon

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

SwissSPAD3 is the latest of a family of widefield time-gated SPAD imagers developed for fluorescence lifetime imaging (FLI) applications. Its distinctive features are (i) the ability to define shorter gates than its predecessors (width W < 1 ns), (ii) support for laser repetition rates up to at least 80 MHz and (iii) a dual-gate architecture providing an effective duty cycle of 100%. We present widefield macroscopic FLI measurements of short lifetime NIR dyes, analyzed using the phasor approach. The results are compared with those previously obtained with SwissSPAD2 and to theoretical predictions.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XXIII
Editors	Ammasi Periasamy, Peter T. So, Karsten Konig
Publisher	SPIE
ISBN (Electronic)	9781510658738
DOIs	https://doi.org/10.1117/12.2649227
State	Published - 2023
Externally published	Yes
Event	Multiphoton Microscopy in the Biomedical Sciences XXIII 2023 - San Francisco, United States Duration: 29 Jan 2023 → 31 Jan 2023

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12384
ISSN (Print)	1605-7422

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XXIII 2023
Country/Territory	United States
City	San Francisco
Period	29/01/23 → 31/01/23

Bibliographical note

Publisher Copyright:
© 2023 SPIE.

Funding

This work was performed as part of a collaborative project between our groups and the Intes Lab at Rensselaer Polytechnic Institute (Troy, NY) and the Barroso Lab at Albany Medical School (Albany, NY) supported by the National Institute of Health Grants R01CA250636. It was supported in part by the Department of Energy grants DESC0020338 and DE-SC0023184. A.U. was supported through the Swiss National Science Foundation under grant 200021-166289.

Funders	Funder number
National Institutes of Health	R01CA250636
U.S. Department of Energy	DE-SC0023184, DESC0020338
Rensselaer Polytechnic Institute
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung	200021-166289

Keywords

NIR
SPAD
fluorescence
lifetime
phasor
time-gated
widefield

Access to Document

10.1117/12.2649227

Cite this

Michalet, X., Ulku, A. C., Wayne, M. A., Weiss, S., Bruschini, C., & Charbon, E. (2023). NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera. In A. Periasamy, P. T. So, & K. Konig (Eds.), Multiphoton Microscopy in the Biomedical Sciences XXIII Article 1238409 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12384). SPIE. https://doi.org/10.1117/12.2649227

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Michalet, X, Ulku, AC, Wayne, MA, Weiss, S, Bruschini, C & Charbon, E 2023, NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera. in A Periasamy, PT So & K Konig (eds), Multiphoton Microscopy in the Biomedical Sciences XXIII., 1238409, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12384, SPIE, Multiphoton Microscopy in the Biomedical Sciences XXIII 2023, San Francisco, United States, 29/01/23. https://doi.org/10.1117/12.2649227

NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera. / Michalet, Xavier; Ulku, Arin C.; Wayne, Michael A. et al.
Multiphoton Microscopy in the Biomedical Sciences XXIII. ed. / Ammasi Periasamy; Peter T. So; Karsten Konig. SPIE, 2023. 1238409 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12384).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera

Abstract

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Keywords

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