Nickel-assisted selective detection of histidine and histidine-rich proteins via an ON-OFF-ON fluorescent probe and its imaging in live cells

Anamika Bandyopadhyay; Adarash Kumar Shukla; Priti Singh; Ashutosh Mahale; Shivkumar Sharma Irukuvajjula; Ramakrishna Vadrevu; Onkar P. Kulkarni; Mudit Dixit; Anupam Bhattacharya

doi:10.1016/j.jphotochem.2023.114885

Nickel-assisted selective detection of histidine and histidine-rich proteins via an ON-OFF-ON fluorescent probe and its imaging in live cells

Anamika Bandyopadhyay, Adarash Kumar Shukla, Priti Singh, Ashutosh Mahale, Shivkumar Sharma Irukuvajjula, Ramakrishna Vadrevu, Onkar P. Kulkarni, Mudit Dixit, Anupam Bhattacharya

Birla Institute of Technology and Science Pilani

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Amino acids and proteins are ubiquitous in all biological processes. Therefore, fluctuations in their levels profoundly impact the general well-being of any human being. Structural similarities between amino acids hamper their direct detection by fluorophores. Thus, it becomes crucial to employ an ancillary approach to accomplish this goal. In this work, the selective fluorescence response of Ni²⁺ towards a probe PyPP was used as a precursor for selective histidine sensing. The probe showed selectivity towards Ni²⁺ by fluorescence quenching, and it could be revived only in the presence of histidine. The limit of detection (LOD) for Ni²⁺ was 4 x10^-6 M, whereas LOD for histidine was 1.08 × 10⁻⁶ M. The Ni²⁺ mediated selectivity could also be extended to histidine-rich proteins bovine serum albumin (BSA) and human serum albumin (HSA). The devised system was also applied to a macrophage cell line (RAW 264.7) to indicate the presence of Ni²⁺/histidine/HSA-BSA via confocal fluorescence microscopy, and quantitative response could be studied with flow cytometry.

Original language	English
Article number	114885
Journal	Journal of Photochemistry and Photobiology A: Chemistry
Volume	443
DOIs	https://doi.org/10.1016/j.jphotochem.2023.114885
State	Published - 1 Sep 2023
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2023 Elsevier B.V.

Funding

The Council for Scientific and Industrial Research (CSIR), New Delhi, is acknowledged for the research grant [Project No. 02(0350)/19/EMR-II]. The authors thank Dr Sridhar Rao Kunchala and Mr T. Chennakesavalu from Oncosimis Biotech Pvt. Ltd. for providing the α-hemolysin sample. The authors would like to thank the central analytical laboratory of the BITS-Pilani Hyderabad campus for help in obtaining the spectroscopic data. The Council for Scientific and Industrial Research (CSIR), New Delhi, is acknowledged for the research grant [Project No. 02(0350)/19/EMR-II]. The authors thank Dr Sridhar Rao Kunchala and Mr T. Chennakesavalu from Oncosimis Biotech Pvt. Ltd. for providing the α-hemolysin sample. The authors would like to thank the central analytical laboratory of the BITS-Pilani Hyderabad campus for help in obtaining the spectroscopic data.

Funders	Funder number
Oncosimis Biotech Pvt. Ltd.
Council of Scientific and Industrial Research, India	02(0350)/19/EMR-II

Keywords

Confocal imaging
FACS
Histidine
Ni sensor
Pyrazolo[1,5-a]pyrimidine
Serum albumin

Access to Document

10.1016/j.jphotochem.2023.114885

Cite this

Bandyopadhyay, A., Kumar Shukla, A., Singh, P., Mahale, A., Sharma Irukuvajjula, S., Vadrevu, R., Kulkarni, O. P., Dixit, M., & Bhattacharya, A. (2023). Nickel-assisted selective detection of histidine and histidine-rich proteins via an ON-OFF-ON fluorescent probe and its imaging in live cells. Journal of Photochemistry and Photobiology A: Chemistry, 443, Article 114885. https://doi.org/10.1016/j.jphotochem.2023.114885

@article{2712d3297dbe471b864bca2589385e7f,

title = "Nickel-assisted selective detection of histidine and histidine-rich proteins via an ON-OFF-ON fluorescent probe and its imaging in live cells",

abstract = "Amino acids and proteins are ubiquitous in all biological processes. Therefore, fluctuations in their levels profoundly impact the general well-being of any human being. Structural similarities between amino acids hamper their direct detection by fluorophores. Thus, it becomes crucial to employ an ancillary approach to accomplish this goal. In this work, the selective fluorescence response of Ni2+ towards a probe PyPP was used as a precursor for selective histidine sensing. The probe showed selectivity towards Ni2+ by fluorescence quenching, and it could be revived only in the presence of histidine. The limit of detection (LOD) for Ni2+ was 4 x10-6 M, whereas LOD for histidine was 1.08 × 10−6 M. The Ni2+ mediated selectivity could also be extended to histidine-rich proteins bovine serum albumin (BSA) and human serum albumin (HSA). The devised system was also applied to a macrophage cell line (RAW 264.7) to indicate the presence of Ni2+/histidine/HSA-BSA via confocal fluorescence microscopy, and quantitative response could be studied with flow cytometry.",

keywords = "Confocal imaging, FACS, Histidine, Ni sensor, Pyrazolo[1,5-a]pyrimidine, Serum albumin",

author = "Anamika Bandyopadhyay and {Kumar Shukla}, Adarash and Priti Singh and Ashutosh Mahale and {Sharma Irukuvajjula}, Shivkumar and Ramakrishna Vadrevu and Kulkarni, {Onkar P.} and Mudit Dixit and Anupam Bhattacharya",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier B.V.",

year = "2023",

month = sep,

day = "1",

doi = "10.1016/j.jphotochem.2023.114885",

language = "אנגלית",

volume = "443",

journal = "Journal of Photochemistry and Photobiology A: Chemistry",

issn = "1010-6030",

publisher = "Elsevier B.V.",

}

Bandyopadhyay, A, Kumar Shukla, A, Singh, P, Mahale, A, Sharma Irukuvajjula, S, Vadrevu, R, Kulkarni, OP, Dixit, M & Bhattacharya, A 2023, 'Nickel-assisted selective detection of histidine and histidine-rich proteins via an ON-OFF-ON fluorescent probe and its imaging in live cells', Journal of Photochemistry and Photobiology A: Chemistry, vol. 443, 114885. https://doi.org/10.1016/j.jphotochem.2023.114885

Nickel-assisted selective detection of histidine and histidine-rich proteins via an ON-OFF-ON fluorescent probe and its imaging in live cells. / Bandyopadhyay, Anamika; Kumar Shukla, Adarash; Singh, Priti et al.
In: Journal of Photochemistry and Photobiology A: Chemistry, Vol. 443, 114885, 01.09.2023.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Nickel-assisted selective detection of histidine and histidine-rich proteins via an ON-OFF-ON fluorescent probe and its imaging in live cells

AU - Bandyopadhyay, Anamika

AU - Kumar Shukla, Adarash

AU - Singh, Priti

AU - Mahale, Ashutosh

AU - Sharma Irukuvajjula, Shivkumar

AU - Vadrevu, Ramakrishna

AU - Kulkarni, Onkar P.

AU - Dixit, Mudit

AU - Bhattacharya, Anupam

PY - 2023/9/1

Y1 - 2023/9/1

N2 - Amino acids and proteins are ubiquitous in all biological processes. Therefore, fluctuations in their levels profoundly impact the general well-being of any human being. Structural similarities between amino acids hamper their direct detection by fluorophores. Thus, it becomes crucial to employ an ancillary approach to accomplish this goal. In this work, the selective fluorescence response of Ni2+ towards a probe PyPP was used as a precursor for selective histidine sensing. The probe showed selectivity towards Ni2+ by fluorescence quenching, and it could be revived only in the presence of histidine. The limit of detection (LOD) for Ni2+ was 4 x10-6 M, whereas LOD for histidine was 1.08 × 10−6 M. The Ni2+ mediated selectivity could also be extended to histidine-rich proteins bovine serum albumin (BSA) and human serum albumin (HSA). The devised system was also applied to a macrophage cell line (RAW 264.7) to indicate the presence of Ni2+/histidine/HSA-BSA via confocal fluorescence microscopy, and quantitative response could be studied with flow cytometry.

AB - Amino acids and proteins are ubiquitous in all biological processes. Therefore, fluctuations in their levels profoundly impact the general well-being of any human being. Structural similarities between amino acids hamper their direct detection by fluorophores. Thus, it becomes crucial to employ an ancillary approach to accomplish this goal. In this work, the selective fluorescence response of Ni2+ towards a probe PyPP was used as a precursor for selective histidine sensing. The probe showed selectivity towards Ni2+ by fluorescence quenching, and it could be revived only in the presence of histidine. The limit of detection (LOD) for Ni2+ was 4 x10-6 M, whereas LOD for histidine was 1.08 × 10−6 M. The Ni2+ mediated selectivity could also be extended to histidine-rich proteins bovine serum albumin (BSA) and human serum albumin (HSA). The devised system was also applied to a macrophage cell line (RAW 264.7) to indicate the presence of Ni2+/histidine/HSA-BSA via confocal fluorescence microscopy, and quantitative response could be studied with flow cytometry.

KW - Confocal imaging

KW - FACS

KW - Histidine

KW - Ni sensor

KW - Pyrazolo[1,5-a]pyrimidine

KW - Serum albumin

UR - http://www.scopus.com/inward/record.url?scp=85163364458&partnerID=8YFLogxK

U2 - 10.1016/j.jphotochem.2023.114885

DO - 10.1016/j.jphotochem.2023.114885

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:85163364458

SN - 1010-6030

VL - 443

JO - Journal of Photochemistry and Photobiology A: Chemistry

JF - Journal of Photochemistry and Photobiology A: Chemistry

M1 - 114885

ER -

Nickel-assisted selective detection of histidine and histidine-rich proteins via an ON-OFF-ON fluorescent probe and its imaging in live cells

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this