Next-generation detector improves single-molecule imaging

Xavier Michalet, Shimon Weiss, Katsushi Arisaka, Dswald H.W. Siegmund, John V. Vallerga, Patrick Jelinsky, Jacques E. Millaud

Research output: Contribution to specialist publicationArticle


A high-throughput next generation three-dimensional detector (H33D) combines the advantages of wide field detectors and high temporal-resolution point detectors, and proved to be effective for single molecule imaging and the study of bimolecular interactions. Samples of dyes with known lifetimes were imaged as thin slabs of liquid between two class coverslips using a fematosecond Ti-sapphire laser tuned at 720 nm, and frequency was doubled using a BBO crystal. The time grating capabilities of the detector were investigated using live cells fused with a particular chemical and detected with quantum dots. H33D detectors use better photo-cathode and readout schemes, which is expected to further improve temporally and spatially resolved spectroscopy and microscopy at the single-molecule level.

Original languageEnglish
Number of pages5
Specialist publicationLaser Focus World
StatePublished - Feb 2007
Externally publishedYes


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