Next-generation detector improves single-molecule imaging

Xavier Michalet; Shimon Weiss; Katsushi Arisaka; Dswald H.W. Siegmund; John V. Vallerga; Patrick Jelinsky; Jacques E. Millaud

Next-generation detector improves single-molecule imaging

Xavier Michalet, Shimon Weiss, Katsushi Arisaka, Dswald H.W. Siegmund, John V. Vallerga, Patrick Jelinsky, Jacques E. Millaud

Research output: Contribution to specialist publication › Article

Abstract

A high-throughput next generation three-dimensional detector (H33D) combines the advantages of wide field detectors and high temporal-resolution point detectors, and proved to be effective for single molecule imaging and the study of bimolecular interactions. Samples of dyes with known lifetimes were imaged as thin slabs of liquid between two class coverslips using a fematosecond Ti-sapphire laser tuned at 720 nm, and frequency was doubled using a BBO crystal. The time grating capabilities of the detector were investigated using live cells fused with a particular chemical and detected with quantum dots. H33D detectors use better photo-cathode and readout schemes, which is expected to further improve temporally and spatially resolved spectroscopy and microscopy at the single-molecule level.

Original language	English
Pages	97-101
Number of pages	5
Volume	43
No	2
Specialist publication	Laser Focus World
State	Published - Feb 2007
Externally published	Yes

Cite this

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title = "Next-generation detector improves single-molecule imaging",

abstract = "A high-throughput next generation three-dimensional detector (H33D) combines the advantages of wide field detectors and high temporal-resolution point detectors, and proved to be effective for single molecule imaging and the study of bimolecular interactions. Samples of dyes with known lifetimes were imaged as thin slabs of liquid between two class coverslips using a fematosecond Ti-sapphire laser tuned at 720 nm, and frequency was doubled using a BBO crystal. The time grating capabilities of the detector were investigated using live cells fused with a particular chemical and detected with quantum dots. H33D detectors use better photo-cathode and readout schemes, which is expected to further improve temporally and spatially resolved spectroscopy and microscopy at the single-molecule level.",

author = "Xavier Michalet and Shimon Weiss and Katsushi Arisaka and Siegmund, {Dswald H.W.} and Vallerga, {John V.} and Patrick Jelinsky and Millaud, {Jacques E.}",

year = "2007",

month = feb,

language = "אנגלית",

volume = "43",

pages = "97--101",

journal = "Laser Focus World",

issn = "1043-8092",

publisher = "Pennwell Corporation",

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T1 - Next-generation detector improves single-molecule imaging

AU - Michalet, Xavier

AU - Weiss, Shimon

AU - Arisaka, Katsushi

AU - Siegmund, Dswald H.W.

AU - Vallerga, John V.

AU - Jelinsky, Patrick

AU - Millaud, Jacques E.

PY - 2007/2

Y1 - 2007/2

N2 - A high-throughput next generation three-dimensional detector (H33D) combines the advantages of wide field detectors and high temporal-resolution point detectors, and proved to be effective for single molecule imaging and the study of bimolecular interactions. Samples of dyes with known lifetimes were imaged as thin slabs of liquid between two class coverslips using a fematosecond Ti-sapphire laser tuned at 720 nm, and frequency was doubled using a BBO crystal. The time grating capabilities of the detector were investigated using live cells fused with a particular chemical and detected with quantum dots. H33D detectors use better photo-cathode and readout schemes, which is expected to further improve temporally and spatially resolved spectroscopy and microscopy at the single-molecule level.

AB - A high-throughput next generation three-dimensional detector (H33D) combines the advantages of wide field detectors and high temporal-resolution point detectors, and proved to be effective for single molecule imaging and the study of bimolecular interactions. Samples of dyes with known lifetimes were imaged as thin slabs of liquid between two class coverslips using a fematosecond Ti-sapphire laser tuned at 720 nm, and frequency was doubled using a BBO crystal. The time grating capabilities of the detector were investigated using live cells fused with a particular chemical and detected with quantum dots. H33D detectors use better photo-cathode and readout schemes, which is expected to further improve temporally and spatially resolved spectroscopy and microscopy at the single-molecule level.

UR - http://www.scopus.com/inward/record.url?scp=33847779944&partnerID=8YFLogxK

M3 - מאמר בכתב עת

AN - SCOPUS:33847779944

SN - 1043-8092

VL - 43

SP - 97

EP - 101

JO - Laser Focus World

JF - Laser Focus World

ER -

Next-generation detector improves single-molecule imaging

Abstract

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