New directions in super resolved microscopy

Tali Ilovitsh, Yossef Danan, Amihai Meiri, Carl G. Ebeling, Zeev Zalevsky

Research output: Contribution to journalConference articlepeer-review

Abstract

We present two new directions in super resolved microscopy. The first uses a modified nonlinear image decomposition technique termed K-factor. It reshapes the 3D point spread function of an XYZ Z-stack images into a Gaussian shape, which simplifies the localization process and its accuracy, thus, the obtained resolution. The second improves the localization accuracy by labeling the specimens with gold nano particles and their subsequent flickering at different temporal frequencies.

Original languageEnglish
Article numberJW3A.1
JournalOptics InfoBase Conference Papers
StatePublished - 2015
EventComputational Optical Sensing and Imaging, COSI 2015 - Arlington, United States
Duration: 7 Jun 201511 Jun 2015

Bibliographical note

Publisher Copyright:
© 2015 OSA.

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