We present two new directions in super resolved microscopy. The first uses a modified nonlinear image decomposition technique termed K-factor. It reshapes the 3D point spread function of an XYZ Z-stack images into a Gaussian shape, which simplifies the localization process and its accuracy, thus, the obtained resolution. The second improves the localization accuracy by labeling the specimens with gold nano particles and their subsequent flickering at different temporal frequencies.
|Journal||Optics InfoBase Conference Papers|
|State||Published - 2015|
|Event||Computational Optical Sensing and Imaging, COSI 2015 - Arlington, United States|
Duration: 7 Jun 2015 → 11 Jun 2015
Bibliographical notePublisher Copyright:
© 2015 OSA.