TY - JOUR
T1 - Neutrophils augment the release of TNFα from LPS-stimulated macrophages via hydrogen peroxide
AU - Sredni-Kenigsbuch, Dvora
AU - Kambayashi, Taku
AU - Strassmann, Gideon
PY - 2000/2/1
Y1 - 2000/2/1
N2 - We examined the effect of polymorphonuclear cells on the release of tumor necrosis factor (TNFα) in endotoxin-treated macrophages. Human peripheral blood neutrophils were co-cultured with mouse peritoneal macrophages stimulated with lipopolysaccharide (LPS). In a dose-dependent manner, FMLP (n-formyl-methionyl-leucyl-phenylalanine) augmented the release of TNFα by LPS-stimulated macrophages in the presence, but not in the absence, of neutrophils. The stimulating effect of neutrophils on macrophages was reversed by catalase, suggesting that the release of hydrogen peroxide from neutrophils was responsible for augmenting macrophage TNFα. Moreover, the direct addition of hydrogen peroxide to macrophages resulted in an increased secretion of TNFα. In addition, insertion of a porous membrane between the neutrophils and macrophages cancelled the effect, indicating that adherence of neutrophils may be necessary for augmentation of TNFα release. In summary, the data suggest that hydrogen peroxide released from stimulated neutrophils may act as an activator of macrophage function by increasing their release of TNFα. (C) 2000 Published by Elsevier Science B.V.
AB - We examined the effect of polymorphonuclear cells on the release of tumor necrosis factor (TNFα) in endotoxin-treated macrophages. Human peripheral blood neutrophils were co-cultured with mouse peritoneal macrophages stimulated with lipopolysaccharide (LPS). In a dose-dependent manner, FMLP (n-formyl-methionyl-leucyl-phenylalanine) augmented the release of TNFα by LPS-stimulated macrophages in the presence, but not in the absence, of neutrophils. The stimulating effect of neutrophils on macrophages was reversed by catalase, suggesting that the release of hydrogen peroxide from neutrophils was responsible for augmenting macrophage TNFα. Moreover, the direct addition of hydrogen peroxide to macrophages resulted in an increased secretion of TNFα. In addition, insertion of a porous membrane between the neutrophils and macrophages cancelled the effect, indicating that adherence of neutrophils may be necessary for augmentation of TNFα release. In summary, the data suggest that hydrogen peroxide released from stimulated neutrophils may act as an activator of macrophage function by increasing their release of TNFα. (C) 2000 Published by Elsevier Science B.V.
KW - Hydrogen peroxide
KW - Macrophages
KW - Tumor necrosis factor-alpha
UR - http://www.scopus.com/inward/record.url?scp=0034141867&partnerID=8YFLogxK
U2 - 10.1016/s0165-2478(99)00157-1
DO - 10.1016/s0165-2478(99)00157-1
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C2 - 10714436
AN - SCOPUS:0034141867
SN - 0165-2478
VL - 71
SP - 97
EP - 102
JO - Immunology Letters
JF - Immunology Letters
IS - 2
ER -