TY - JOUR
T1 - Nerve growth factor signal transduction in human B lymphocytes is mediated by gp140(trk)
AU - Melamed, Isaac
AU - Kelleher, Colm A.
AU - Franklin, Richard A.
AU - Brodie, Chaya
AU - Hempstead, Barbara
AU - Kaplan, David
AU - Gelfand, Erwin W.
PY - 1996/9
Y1 - 1996/9
N2 - Nerve growth factor (NGF) plays an important role in the regulation of the immune system. Recent studies from this laboratory demonstrated the presence of functional NGF receptors on human B lymphocytes; in addition, NGF has been shown to enhance B lymphocyte proliferation. NGF caused both concentration- and lime-dependent increases in tyrosine phosphorylation of five proteins of 140, 110, 85, 60 and 42 kDa, which were identified as phospholipase C-γ1, phosphatidylinositol-3 kinase and mitogen-activated protein kinase. To elucidate the contribution of the Trk family of tyrosine kinases to the phosphorylation events induced by NGF we identified gp140(trk) in human B cells and in human B cell lines. Analysis of specific gp140(trk) immunoprecipitates indicated that addition of NGF to B cells induced a rapid increase in the tyrosine phosphorylation of gp140(trk) and inhibition of this phosphorylation prevented the tyrosine phosphorylation of other proteins. These data identify the central role of gp140(trk) in NGF signaling of human B lymphocytes.
AB - Nerve growth factor (NGF) plays an important role in the regulation of the immune system. Recent studies from this laboratory demonstrated the presence of functional NGF receptors on human B lymphocytes; in addition, NGF has been shown to enhance B lymphocyte proliferation. NGF caused both concentration- and lime-dependent increases in tyrosine phosphorylation of five proteins of 140, 110, 85, 60 and 42 kDa, which were identified as phospholipase C-γ1, phosphatidylinositol-3 kinase and mitogen-activated protein kinase. To elucidate the contribution of the Trk family of tyrosine kinases to the phosphorylation events induced by NGF we identified gp140(trk) in human B cells and in human B cell lines. Analysis of specific gp140(trk) immunoprecipitates indicated that addition of NGF to B cells induced a rapid increase in the tyrosine phosphorylation of gp140(trk) and inhibition of this phosphorylation prevented the tyrosine phosphorylation of other proteins. These data identify the central role of gp140(trk) in NGF signaling of human B lymphocytes.
KW - B lymphocyte
KW - Nerve growth factor
KW - Nerve growth factor receptor
KW - Tyrosine kinase
KW - gp140(trk)
UR - http://www.scopus.com/inward/record.url?scp=0029836783&partnerID=8YFLogxK
U2 - 10.1002/eji.1830260903
DO - 10.1002/eji.1830260903
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C2 - 8814235
SN - 0014-2980
VL - 26
SP - 1985
EP - 1992
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 9
ER -