Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression

Nissan Yissachar; Hagit Salem; Tamar Tennenbaum; Benny Motro

doi:10.1016/j.febslet.2006.10.069

Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression

Nissan Yissachar, Hagit Salem, Tamar Tennenbaum, Benny Motro

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Members of the NIMA-related kinases (NRK) family are recently emerging as central regulators of various aspects of the cell cycle. However, the cellular roles of the mammalian NRK, Nek7, remain obscure. We show here that the endogenous Nek7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective Nek7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells. Down-regulation of Nek7 using a small interfering RNA approach resulted in a significant increase in mitotic cells presenting multipolar spindle phenotype. These results suggest a role for Nek7 in regulating proper spindle assembly and mitotic progression.

Original language	English
Pages (from-to)	6489-6495
Number of pages	7
Journal	FEBS Letters
Volume	580
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2006.10.069
State	Published - 27 Nov 2006

Bibliographical note

Funding Information:
We thank Dr. Salisbury, Mayo Clinic, Rochester, for kindly providing Centrin-GFP expression vector. This study was supported by Grant No. 5508 from the Chief Scientist’s Office of the Ministry of Health, Israel.

Keywords

Centrosome
Chromosome segregation
Kinase
Multipolar spindle
NIMA

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10.1016/j.febslet.2006.10.069

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title = "Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression",

abstract = "Members of the NIMA-related kinases (NRK) family are recently emerging as central regulators of various aspects of the cell cycle. However, the cellular roles of the mammalian NRK, Nek7, remain obscure. We show here that the endogenous Nek7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective Nek7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells. Down-regulation of Nek7 using a small interfering RNA approach resulted in a significant increase in mitotic cells presenting multipolar spindle phenotype. These results suggest a role for Nek7 in regulating proper spindle assembly and mitotic progression.",

keywords = "Centrosome, Chromosome segregation, Kinase, Multipolar spindle, NIMA",

author = "Nissan Yissachar and Hagit Salem and Tamar Tennenbaum and Benny Motro",

note = "Funding Information: We thank Dr. Salisbury, Mayo Clinic, Rochester, for kindly providing Centrin-GFP expression vector. This study was supported by Grant No. 5508 from the Chief Scientist{\textquoteright}s Office of the Ministry of Health, Israel.",

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AU - Salem, Hagit

AU - Tennenbaum, Tamar

AU - Motro, Benny

N1 - Funding Information: We thank Dr. Salisbury, Mayo Clinic, Rochester, for kindly providing Centrin-GFP expression vector. This study was supported by Grant No. 5508 from the Chief Scientist’s Office of the Ministry of Health, Israel.

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AB - Members of the NIMA-related kinases (NRK) family are recently emerging as central regulators of various aspects of the cell cycle. However, the cellular roles of the mammalian NRK, Nek7, remain obscure. We show here that the endogenous Nek7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective Nek7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells. Down-regulation of Nek7 using a small interfering RNA approach resulted in a significant increase in mitotic cells presenting multipolar spindle phenotype. These results suggest a role for Nek7 in regulating proper spindle assembly and mitotic progression.

KW - Centrosome

KW - Chromosome segregation

KW - Kinase

KW - Multipolar spindle

KW - NIMA

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Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression

Abstract

Bibliographical note

Keywords

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