The Aspergillus NIMA serine/threonine kinase plays a pivotal role in controlling entrance into mitosis. A major function attributed to NIMA is the induction of chromatin condensation. We show here that the founder murine NIMA-related kinase, Nek1, is larger than previously reported, and that the full-length protein conserves the structural hallmarks of NIMA. Even though Nek1 bears two classical nuclear localization signals (NLS), the endogenous protein localizes to the cytoplasm. Ectopic overexpression of various Nek1 constructs suggests that the C-terminus of Nek1 bears cytoplasmic localization signal(s). Overexpression of nuclear constructs of Nek1 resulted in abnormal chromatin condensation, with the DNA mainly confined to the periphery of the nucleus. Advanced condensation phenotype was associated with nuclear pore complex dispersal. The condensation was not accompanied by up-regulation of mitotic or apoptotic markers. A similar phenotype has been described following NIMA overexpression, strengthening the notion that the mammalian Nek1 kinase has functional similarity to NIMA.
|Number of pages||10|
|Journal||Biochimica et Biophysica Acta - Molecular Cell Research|
|State||Published - Mar 2006|
Bibliographical noteFunding Information:
We thank Dr. Poornima Upadhya for providing kat mutant mice, and Dr. Richard Wozniak, University of Alberta, for Nup53-GFP construct. This work was supported by the Israel Cancer Research Fund.
- Cell cycle
- Chromatin condensation
- Nek kinase
- Sub-cellular localization