Near-field fluorescence microscopy of cells

Th Enderle, T. Ha, D. S. Chemla, S. Weiss

Research output: Contribution to journalConference articlepeer-review

44 Scopus citations

Abstract

We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins.

Original languageEnglish
Pages (from-to)303-309
Number of pages7
JournalUltramicroscopy
Volume71
Issue number1-4
DOIs
StatePublished - 1 Mar 1998
Externally publishedYes
EventProceedings of the 1997 4th International Conference on Near-Field Optics and Related Techniques, NFO-4 - Jerusalem, Israel
Duration: 9 Feb 199713 Feb 1997

Bibliographical note

Funding Information:
We thank J. Maguire, C.D. Webb and R. Losick for providing the Bacillus subtilis samples and C. Magowan for providing the malaria-infected red-blood cells samples. We gratefully acknowledge the loan of the Innova 70 Spectrum laser from Coherent, Inc., Laser Group, Santa Clara, California. This work was supported by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory under the US Department of Energy, Contract No. DE-AC03-76SF00098 and by the Office of Naval Research, Contract No. N00014-95-F-0099.

Funding

We thank J. Maguire, C.D. Webb and R. Losick for providing the Bacillus subtilis samples and C. Magowan for providing the malaria-infected red-blood cells samples. We gratefully acknowledge the loan of the Innova 70 Spectrum laser from Coherent, Inc., Laser Group, Santa Clara, California. This work was supported by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory under the US Department of Energy, Contract No. DE-AC03-76SF00098 and by the Office of Naval Research, Contract No. N00014-95-F-0099.

FundersFunder number
US Department of EnergyDE-AC03-76SF00098
Office of Naval Research
Lawrence Berkeley National Laboratory

    Keywords

    • Green fluorescent protein
    • Malaria
    • Microscopic methods, specifically for biological specimens
    • Near-field optical microscopy
    • Red- blood cells
    • Spectroscopy in NFOM

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