We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins.
|Number of pages||7|
|State||Published - 1 Mar 1998|
|Event||Proceedings of the 1997 4th International Conference on Near-Field Optics and Related Techniques, NFO-4 - Jerusalem, Israel|
Duration: 9 Feb 1997 → 13 Feb 1997
Bibliographical noteFunding Information:
We thank J. Maguire, C.D. Webb and R. Losick for providing the Bacillus subtilis samples and C. Magowan for providing the malaria-infected red-blood cells samples. We gratefully acknowledge the loan of the Innova 70 Spectrum laser from Coherent, Inc., Laser Group, Santa Clara, California. This work was supported by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory under the US Department of Energy, Contract No. DE-AC03-76SF00098 and by the Office of Naval Research, Contract No. N00014-95-F-0099.
- Green fluorescent protein
- Microscopic methods, specifically for biological specimens
- Near-field optical microscopy
- Red- blood cells
- Spectroscopy in NFOM