Nanoscale spatially resolved infrared spectra from single microdroplets

Thomas Müller, Francesco Simone Ruggeri, Andrzej J. Kulik, Ulyana Shimanovich, Thomas O. Mason, Tuomas P.J. Knowles, Giovanni Dietler

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Droplet microfluidics has emerged as a powerful platform allowing a large number of individual reactions to be carried out in spatially distinct microcompartments. Due to their small size, however, the spectroscopic characterisation of species encapsulated in such systems remains challenging. In this paper, we demonstrate the acquisition of infrared spectra from single microdroplets containing aggregation-prone proteins. To this effect, droplets are generated in a microfluidic flow-focussing device and subsequently deposited in a square array onto a ZnSe prism using a micro stamp. After drying, the solutes present in the droplets are illuminated locally by an infrared laser through the prism, and their thermal expansion upon absorption of infrared radiation is measured with an atomic force microscopy tip, granting nanoscale resolution. Using this approach, we resolve structural differences in the amide bands of the spectra of monomeric and aggregated lysozyme from single microdroplets with picolitre volume.

Original languageEnglish
Pages (from-to)1315-1319
Number of pages5
JournalLab on a Chip
Issue number7
StatePublished - 7 Apr 2014
Externally publishedYes


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