Nanoparticle Mobility over a Surface as a Probe for Weak Transient Disordered Peptide-Peptide Interactions

Indrani Chakraborty; Gil Rahamim; Ram Avinery; Yael Roichman; Roy Beck

doi:10.1021/acs.nanolett.9b02764

Nanoparticle Mobility over a Surface as a Probe for Weak Transient Disordered Peptide-Peptide Interactions

Indrani Chakraborty, Gil Rahamim, Ram Avinery, Yael Roichman, Roy Beck

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Weak interactions form the core basis of a vast number of biological processes, in particular, those involving intrinsically disordered proteins. Here, we establish a new technique capable of probing these weak interactions between synthetic unfolded polypeptides using a convenient yet efficient, quantitative method based on single particle tracking of peptide-coated gold nanoparticles over peptide-coated surfaces. We demonstrate that our technique is sensitive enough to observe the influence of a single amino acid mutation on the transient peptide-peptide interactions. Furthermore, the effects of buffer salinity, which are expected to alter weak electrostatic interactions, are also readily detected and examined in detail. The method presented here has the potential to evaluate, in a high-throughput manner, weak interactions for a wide range of disordered proteins, polypeptides, and other biomolecules.

Original language	English
Pages (from-to)	6524-6534
Number of pages	11
Journal	Nano Letters
Volume	19
Issue number	9
DOIs	https://doi.org/10.1021/acs.nanolett.9b02764
State	Published - 11 Sep 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 American Chemical Society.

Funding

I.C. prepared the glass surfaces, assembled the sample chambers, and performed the particle tracking experiments and analysis. G.R. and R.A. designed the sequences. G.R. functionalized the GNPs with peptides and measured grafting densities. R.A. conceived the basic concept of the project. R.B. and Y.R. lead the project. All the authors designed the experiments and participated in writing the paper. This work was supported by the Israel Science foundation (ISF) (Grant Nos. 453/17, 550/15, and 988/17) and the United States–Israel Binational Science Foundation (No. 201696). The authors declare no competing financial interest. I.C. acknowledges the PBC Postdoctoral fellowship of the Council for Higher Education of Israel. The authors acknowledge helpful discussions with Fernando Patolsky, Yacov Kantor, Micha Kornreich, and Guy Jacoby.

Funders	Funder number
I.C.
United States-Israel Binational Science Foundation	201696
Israel Science Foundation	550/15, 453/17, 988/17
Council for Higher Education
Planning and Budgeting Committee of the Council for Higher Education of Israel

Keywords

diffusion
gold nanoparticles
intrinsically disordered proteins
particle tracking
peptide-peptide interactions
weak interactions

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10.1021/acs.nanolett.9b02764

Cite this

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Nanoparticle Mobility over a Surface as a Probe for Weak Transient Disordered Peptide-Peptide Interactions

Abstract

Bibliographical note

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Keywords

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