Nanoparticle Mobility over a Surface as a Probe for Weak Transient Disordered Peptide-Peptide Interactions

Indrani Chakraborty, Gil Rahamim, Ram Avinery, Yael Roichman, Roy Beck

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Weak interactions form the core basis of a vast number of biological processes, in particular, those involving intrinsically disordered proteins. Here, we establish a new technique capable of probing these weak interactions between synthetic unfolded polypeptides using a convenient yet efficient, quantitative method based on single particle tracking of peptide-coated gold nanoparticles over peptide-coated surfaces. We demonstrate that our technique is sensitive enough to observe the influence of a single amino acid mutation on the transient peptide-peptide interactions. Furthermore, the effects of buffer salinity, which are expected to alter weak electrostatic interactions, are also readily detected and examined in detail. The method presented here has the potential to evaluate, in a high-throughput manner, weak interactions for a wide range of disordered proteins, polypeptides, and other biomolecules.

Original languageEnglish
Pages (from-to)6524-6534
Number of pages11
JournalNano Letters
Volume19
Issue number9
DOIs
StatePublished - 11 Sep 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 American Chemical Society.

Keywords

  • diffusion
  • gold nanoparticles
  • intrinsically disordered proteins
  • particle tracking
  • peptide-peptide interactions
  • weak interactions

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