Nanoparticle-amplified surface plasmon resonance for detection of DNA hybridization

G. P. Goodrich, S. R. Nicewarner, L. He, M. J. Natan, C. D. Keating

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations


In recent years there has been a great deal of interest in the measurement of DNA hybridization at surfaces. Surface-confined DNA hybridization has been used to monitor gene expression, to detect the presence of a particular DNA sequence and determine single nucleotide polymorphisms (SNPs). DNA microarrays, which can contain thousands of discrete DNA sequences on a single surface, have become widely used for hybridization studies. While a powerful technique, this technology is limited by the stability of the fluorescent dyes used to label the DNA, and the need to perform measurements ex-situ to reduce the fluorescence background. In this report, we describe the use of colloid-amplified surface plasmon resonance (SPR) to measure DNA hybridization at surfaces. SPR is a surface sensitive technique, which can be used to study hybridization in situ, and the use of colloidal metal tags provides excellent sensitivity. Angle-scanning SPR has been used to study oligonucleotide hybridization to surface confined probes, and work is underway to apply SPR imaging to study DNA hybridization in macro-and microarray formats.

Original languageEnglish
Pages (from-to)80-85
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2001
Externally publishedYes
EventNanoparticles and Nanostrutured Surfaces: Novel Reporters with Biological Applications - San Jose, CA, United States
Duration: 24 Jan 200125 Jan 2001


  • Au colloid
  • DNA
  • Hybridization
  • Nanoparticle
  • Surface plasmon resonance


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