Nanometer distance measurements between multicolor quantum dots

Josh Antelman, Connie Wilking-Chang, Shimon Weiss, Xavier Michalet

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Quantum dot dimers made of short double-stranded DNA molecules labeled with different color quantum dots at each end were imaged using multicolor stage-scanning confocal microscopy. This approach eliminates chromatic aberration and color registration issues usually encountered in other multicolor imaging techniques. We demonstrate nanometer accuracy in individual distance measurement by suppression of quantum dot blinking and thoroughly characterize the contribution of different effects to the variability observed between measurements. Our analysis opens the way to accurate structural studies of biomolecules and biomolecular complexes using multicolor quantum labeling.

Original languageEnglish
Pages (from-to)2199-2205
Number of pages7
JournalNano Letters
Volume9
Issue number5
DOIs
StatePublished - 13 May 2009
Externally publishedYes

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM069709
National Institute of Biomedical Imaging and BioengineeringR01EB000312

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