Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro

M. Satyanarayana; K. Koteshwara Reddy; K. Vengatajalabathy Gobi

doi:10.1002/elan.201400243

Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro

M. Satyanarayana
, K. Koteshwara Reddy
, K. Vengatajalabathy Gobi

National Institute of Technology Warangal

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

A multiwalled carbon nanotube - chitosan (MWCNT-Chit) composite modified glassy carbon electrode (GCE) was fabricated and applied for sensitive and selective determination of serotonin also known as 5-hydroxytryptamine (5-HT). Electroanalytical response of the fabricated electrode towards the detection of 5-HT was studied by using cyclic voltammetry and electrochemical impedance spectroscopy. The results show that the MWCNT-Chit/GCE has greatly enhanced the oxidation peak current and that the nanocomposite film enhanced the electron transfer to the analyte. Applying differential pulse voltammetry method, a linear calibration plot has been achieved over the concentration range 5×10^-8-1.6×10^-5M and the low-detection-limit is 5×10^-8M. Selective determination of serotonin in the presence of dopamine, ascorbic acid and uric acid was established. The fabricated biosensor detects 5-HT in-vitro directly from artificial urine samples and from diluted serum samples.

Original language	English
Pages (from-to)	2365-2372
Number of pages	8
Journal	Electroanalysis
Volume	26
Issue number	11
DOIs	https://doi.org/10.1002/elan.201400243
State	Published - 1 Nov 2014
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

Artificial urine
Carbon nanotubes
Chitosan
Electrocatalysis
Serotonin
Voltammetric determination

Access to Document

10.1002/elan.201400243

Cite this

@article{8a20499ed4d64f6a9284dd201564d057,

title = "Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro",

abstract = "A multiwalled carbon nanotube - chitosan (MWCNT-Chit) composite modified glassy carbon electrode (GCE) was fabricated and applied for sensitive and selective determination of serotonin also known as 5-hydroxytryptamine (5-HT). Electroanalytical response of the fabricated electrode towards the detection of 5-HT was studied by using cyclic voltammetry and electrochemical impedance spectroscopy. The results show that the MWCNT-Chit/GCE has greatly enhanced the oxidation peak current and that the nanocomposite film enhanced the electron transfer to the analyte. Applying differential pulse voltammetry method, a linear calibration plot has been achieved over the concentration range 5×10-8-1.6×10-5M and the low-detection-limit is 5×10-8M. Selective determination of serotonin in the presence of dopamine, ascorbic acid and uric acid was established. The fabricated biosensor detects 5-HT in-vitro directly from artificial urine samples and from diluted serum samples.",

keywords = "Artificial urine, Carbon nanotubes, Chitosan, Electrocatalysis, Serotonin, Voltammetric determination",

author = "M. Satyanarayana and \{Koteshwara Reddy\}, K. and \{Vengatajalabathy Gobi\}, K.",

note = "Publisher Copyright: {\textcopyright} 2014 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.",

year = "2014",

month = nov,

day = "1",

doi = "10.1002/elan.201400243",

language = "אנגלית",

volume = "26",

pages = "2365--2372",

journal = "Electroanalysis",

issn = "1040-0397",

publisher = "Wiley-VCH Verlag",

number = "11",

}

TY - JOUR

T1 - Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro

AU - Satyanarayana, M.

AU - Koteshwara Reddy, K.

AU - Vengatajalabathy Gobi, K.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - A multiwalled carbon nanotube - chitosan (MWCNT-Chit) composite modified glassy carbon electrode (GCE) was fabricated and applied for sensitive and selective determination of serotonin also known as 5-hydroxytryptamine (5-HT). Electroanalytical response of the fabricated electrode towards the detection of 5-HT was studied by using cyclic voltammetry and electrochemical impedance spectroscopy. The results show that the MWCNT-Chit/GCE has greatly enhanced the oxidation peak current and that the nanocomposite film enhanced the electron transfer to the analyte. Applying differential pulse voltammetry method, a linear calibration plot has been achieved over the concentration range 5×10-8-1.6×10-5M and the low-detection-limit is 5×10-8M. Selective determination of serotonin in the presence of dopamine, ascorbic acid and uric acid was established. The fabricated biosensor detects 5-HT in-vitro directly from artificial urine samples and from diluted serum samples.

AB - A multiwalled carbon nanotube - chitosan (MWCNT-Chit) composite modified glassy carbon electrode (GCE) was fabricated and applied for sensitive and selective determination of serotonin also known as 5-hydroxytryptamine (5-HT). Electroanalytical response of the fabricated electrode towards the detection of 5-HT was studied by using cyclic voltammetry and electrochemical impedance spectroscopy. The results show that the MWCNT-Chit/GCE has greatly enhanced the oxidation peak current and that the nanocomposite film enhanced the electron transfer to the analyte. Applying differential pulse voltammetry method, a linear calibration plot has been achieved over the concentration range 5×10-8-1.6×10-5M and the low-detection-limit is 5×10-8M. Selective determination of serotonin in the presence of dopamine, ascorbic acid and uric acid was established. The fabricated biosensor detects 5-HT in-vitro directly from artificial urine samples and from diluted serum samples.

KW - Artificial urine

KW - Carbon nanotubes

KW - Chitosan

KW - Electrocatalysis

KW - Serotonin

KW - Voltammetric determination

UR - https://www.scopus.com/pages/publications/84910609576

U2 - 10.1002/elan.201400243

DO - 10.1002/elan.201400243

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:84910609576

SN - 1040-0397

VL - 26

SP - 2365

EP - 2372

JO - Electroanalysis

JF - Electroanalysis

IS - 11

ER -

Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this