Myogenesis on microcarrier cultures

A. Shainberg; A. Isac; S. Reuveny; A. Mizrahi; A. Shahar

doi:10.1016/0309-1651(83)90200-x

Myogenesis on microcarrier cultures

A. Shainberg
, A. Isac
, S. Reuveny
, A. Mizrahi
, A. Shahar

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Bar-Ilan University
Israel Institute for Biological Research

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The capacity of embryonic chick myoblasts to grow in vitro on DEAE-cellulose microcarriers (MC) has been investigated biochemically and morphologically. The cells attached to the MC, replicated and fused to form elongated myotubes. These myotubes synthesized muscle-specific proteins, such as creatine kinase (CK) and acetylcholine receptors (AChR), and they contracted spontaneously. Some of the advantages of this technique are: a) Tridimensional development of myotubes on MC with orientation of fibers parallel to each other; b) Muscle cells can be cultured on MC for long periods (months); c) Easy harvesting of samples at any time during cultivation; d) DEAE-cellulose MC are commercially available, inexpensive and easy to handle.

Original language	English
Pages (from-to)	727-734
Number of pages	8
Journal	Cell Biology International Reports
Volume	7
Issue number	9
DOIs	https://doi.org/10.1016/0309-1651(83)90200-x
State	Published - Sep 1983

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title = "Myogenesis on microcarrier cultures",

abstract = "The capacity of embryonic chick myoblasts to grow in vitro on DEAE-cellulose microcarriers (MC) has been investigated biochemically and morphologically. The cells attached to the MC, replicated and fused to form elongated myotubes. These myotubes synthesized muscle-specific proteins, such as creatine kinase (CK) and acetylcholine receptors (AChR), and they contracted spontaneously. Some of the advantages of this technique are: a) Tridimensional development of myotubes on MC with orientation of fibers parallel to each other; b) Muscle cells can be cultured on MC for long periods (months); c) Easy harvesting of samples at any time during cultivation; d) DEAE-cellulose MC are commercially available, inexpensive and easy to handle.",

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AU - Shainberg, A.

AU - Isac, A.

AU - Reuveny, S.

AU - Mizrahi, A.

AU - Shahar, A.

PY - 1983/9

Y1 - 1983/9

N2 - The capacity of embryonic chick myoblasts to grow in vitro on DEAE-cellulose microcarriers (MC) has been investigated biochemically and morphologically. The cells attached to the MC, replicated and fused to form elongated myotubes. These myotubes synthesized muscle-specific proteins, such as creatine kinase (CK) and acetylcholine receptors (AChR), and they contracted spontaneously. Some of the advantages of this technique are: a) Tridimensional development of myotubes on MC with orientation of fibers parallel to each other; b) Muscle cells can be cultured on MC for long periods (months); c) Easy harvesting of samples at any time during cultivation; d) DEAE-cellulose MC are commercially available, inexpensive and easy to handle.

AB - The capacity of embryonic chick myoblasts to grow in vitro on DEAE-cellulose microcarriers (MC) has been investigated biochemically and morphologically. The cells attached to the MC, replicated and fused to form elongated myotubes. These myotubes synthesized muscle-specific proteins, such as creatine kinase (CK) and acetylcholine receptors (AChR), and they contracted spontaneously. Some of the advantages of this technique are: a) Tridimensional development of myotubes on MC with orientation of fibers parallel to each other; b) Muscle cells can be cultured on MC for long periods (months); c) Easy harvesting of samples at any time during cultivation; d) DEAE-cellulose MC are commercially available, inexpensive and easy to handle.

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Myogenesis on microcarrier cultures

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