TY - JOUR
T1 - Myogenesis on microcarrier cultures
AU - Shainberg, A.
AU - Isac, A.
AU - Reuveny, S.
AU - Mizrahi, A.
AU - Shahar, A.
PY - 1983/9
Y1 - 1983/9
N2 - The capacity of embryonic chick myoblasts to grow in vitro on DEAE-cellulose microcarriers (MC) has been investigated biochemically and morphologically. The cells attached to the MC, replicated and fused to form elongated myotubes. These myotubes synthesized muscle-specific proteins, such as creatine kinase (CK) and acetylcholine receptors (AChR), and they contracted spontaneously. Some of the advantages of this technique are: a) Tridimensional development of myotubes on MC with orientation of fibers parallel to each other; b) Muscle cells can be cultured on MC for long periods (months); c) Easy harvesting of samples at any time during cultivation; d) DEAE-cellulose MC are commercially available, inexpensive and easy to handle.
AB - The capacity of embryonic chick myoblasts to grow in vitro on DEAE-cellulose microcarriers (MC) has been investigated biochemically and morphologically. The cells attached to the MC, replicated and fused to form elongated myotubes. These myotubes synthesized muscle-specific proteins, such as creatine kinase (CK) and acetylcholine receptors (AChR), and they contracted spontaneously. Some of the advantages of this technique are: a) Tridimensional development of myotubes on MC with orientation of fibers parallel to each other; b) Muscle cells can be cultured on MC for long periods (months); c) Easy harvesting of samples at any time during cultivation; d) DEAE-cellulose MC are commercially available, inexpensive and easy to handle.
UR - http://www.scopus.com/inward/record.url?scp=0020825076&partnerID=8YFLogxK
U2 - 10.1016/0309-1651(83)90200-x
DO - 10.1016/0309-1651(83)90200-x
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C2 - 6627408
AN - SCOPUS:0020825076
SN - 0309-1651
VL - 7
SP - 727
EP - 734
JO - Cell Biology International Reports
JF - Cell Biology International Reports
IS - 9
ER -