TY - JOUR
T1 - Murine peripheral NK-cell populations originate from site-specific immature NK cells more than from BM-derived NK cells
AU - Pinhas, Nissim
AU - Sternberg-Simon, Michal
AU - Chiossone, Laura
AU - Shahaf, Gitit
AU - Walzer, Thierry
AU - Vivier, Eric
AU - Mehr, Ramit
N1 - Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Murine NK cells can be divided by the expression of two cell surface markers, CD27 and Mac-1 (a.k.a. CD11b), into four separate subsets. These subsets suggest a linear development model: CD27- Mac-1- → CD27+ Mac-1- → CD27+ Mac-1+ → CD27- Mac-1+. Here, we used a combination of BrdU labeling experiments and mathematical modeling to gain insights regarding NK-cell development in mouse bone marrow (BM), spleen and liver. The modeling results that best fit the experimental data show that the majority of NK cells already express CD27 upon entering the NK-cell developmental pathway. Additionally, only a small fraction of NK cells exit the BM to other sites, suggesting that peripheral NK-cell populations originate from site-specific immature NK cells more than from BM-derived mature NK cells.
AB - Murine NK cells can be divided by the expression of two cell surface markers, CD27 and Mac-1 (a.k.a. CD11b), into four separate subsets. These subsets suggest a linear development model: CD27- Mac-1- → CD27+ Mac-1- → CD27+ Mac-1+ → CD27- Mac-1+. Here, we used a combination of BrdU labeling experiments and mathematical modeling to gain insights regarding NK-cell development in mouse bone marrow (BM), spleen and liver. The modeling results that best fit the experimental data show that the majority of NK cells already express CD27 upon entering the NK-cell developmental pathway. Additionally, only a small fraction of NK cells exit the BM to other sites, suggesting that peripheral NK-cell populations originate from site-specific immature NK cells more than from BM-derived mature NK cells.
KW - CD11b
KW - CD27
KW - Computer simulation
KW - Mathematical model
KW - Natural killer cells
UR - http://www.scopus.com/inward/record.url?scp=84962656171&partnerID=8YFLogxK
U2 - 10.1002/eji.201545847
DO - 10.1002/eji.201545847
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C2 - 26919267
SN - 0014-2980
VL - 46
SP - 1258
EP - 1270
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 5
ER -