Abstract
The solvatochromic IR responsivity of small side chain –NCS in two unexplored unnatural amino acids, isothiocyanyl alanine (NCSAla = Ita) and lysine (NCSLys = Itl), without perturbing the conformation is demonstrated in two designed short tripeptide (BocAla-NCSAla-Ala-OMe) and hexapeptide (BocLeu-Val-Phe-Phe-NCSLys-Gly-OMe). Demonstration of site specific fluorescent labeling in both the peptides and ligation type reaction in NCSLys indicates the novelty of these two amino acids as alternative to the available canonical amino acids.
Original language | English |
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Pages (from-to) | 1404-1409 |
Number of pages | 6 |
Journal | Bioorganic and Medicinal Chemistry Letters |
DOIs | |
State | Published - 1 May 2018 |
Externally published | Yes |
Bibliographical note
Funding Information:The authors thank the Department of Biotechnology (DBT: BT/PR16620/NER/95/223/2015), New Delhi, Govt. of India for generous funding. S.D. thanks the IIT Guwahati for a fellowship. The CIF, IITG is gratefully acknowledged for 600 MHz NMR facility.
Funding Information:
The authors thank the Department of Biotechnology (DBT: BT/PR16620/NER/95/223/2015), New Delhi, Govt. of India for generous funding. S.D. thanks the IIT Guwahati for a fellowship. The CIF, IITG is gratefully acknowledged for 600 MHz NMR facility.
Publisher Copyright:
© 2018
Keywords
- IR sensitive probe
- Isothiocyanyl alanine
- Isothiocyanyl lysine
- Peptide ligation
- Site specific labeling