Multiple pathways regulating the calorie restriction response in yeast

Ofer Rahat; Noam Maoz; Haim Y. Cohen

doi:10.1093/gerona/glq165

Multiple pathways regulating the calorie restriction response in yeast

Ofer Rahat, Noam Maoz, Haim Y. Cohen

The Mina and Everard Goodman Faculty of Life Sciences

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

In yeast, SIR2 overexpression or calorie restriction (CR) results in life-span extension. It was previously suggested that CR activates Sir2 by reducing the levels of Sir2 inhibitors, NADH, or nicotinamide. Whereas NADH reduction is associated with an increase in respiration, nicotinamide clearance is induced by the upregulation of PNC1. Here, we show that, consistent with the hormesis hypothesis, PNC1 is part of a transcriptional stress response module consisting of 39 genes that increases under various stresses. Under high CR (0.1% glucose), Pnc1 becomes activated and its levels increase. However, low CR (0.5% glucose) increases yeast life span without PNC1 induction or activation of any transcriptional stress response. Instead, microarray analysis of low CR shows that the messenger RNA levels of iron transport genes increase, suggesting that this mode of CR is regulated by a shift toward respiration and lowering NADH levels. Thus, at least two pathways regulate the CR response in yeast.

Original language	English
Pages (from-to)	163-169
Number of pages	7
Journal	Journals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume	66 A
Issue number	2
DOIs	https://doi.org/10.1093/gerona/glq165
State	Published - Feb 2011

Bibliographical note

Funding Information:
This study was supported by grants from the Israeli Academy of Sciences, Binational US-Israel Science Foundation, Israel Cancer Association, Koret Foundation, the Israel Cancer Research Fund, the Israel Health Ministry, and the ERC: European Research Council. O.R. is funded by the Council for Higher Education fellowship.

Funding

This study was supported by grants from the Israeli Academy of Sciences, Binational US-Israel Science Foundation, Israel Cancer Association, Koret Foundation, the Israel Cancer Research Fund, the Israel Health Ministry, and the ERC: European Research Council. O.R. is funded by the Council for Higher Education fellowship.

Funders	Funder number
Israeli Academy of Sciences
Koret Foundation
Israel Cancer Research Fund
European Commission
Israel Cancer Association
Israel Science Foundation
Council for Higher Education
Ministry of Health, State of Israel

Keywords

Calorie restriction
Respiration
Stress
Yeast

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10.1093/gerona/glq165

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Multiple pathways regulating the calorie restriction response in yeast

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