Multiparametric evaluation of brain functions in the mongolian gerbil in vivo

A. Mayevsky, K. Frank, M. Muck, M. Kessler, S. Nioka, B. Chance

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    37 Scopus citations

    Abstract

    We have developed the multiprobe assembly (MPA) by which metabolic, ionic and electrical activities can be monitored from the surface of the brain. In the present study we included optical fibers for the monitoring of intracapillary hemoglobin oxygenation by use of the Erlangen Microlight Guide Spectrophotometer (EMPHO-I) from the surface of the gerbil brain. The newly developed MPA provides simultaneous information about oxygen delivery (oxy-deoxy Hb), tissue pC2 level, as well as the intracellular oxygen balance (intramitochondrial redox state). The ionic homeostasis was evaluated by monitoring extracellular K+ and Ca2+ activities reflecting the permeability changes of cation channels as well as the activities of Na+,K+-ATPase and other ion linked transport proces-ses. The electrical activities were monitored by a bipolar electro-cortical surface probe and DC steady potential. The subjects of the present study were Mongolian gerbils (Meriones unguiculatus) anesthetized and operated according to our routine techniques. After 30 min of recovery from the operation each gerbil was exposed to a short anoxia, graded hypoxia, ischemia as well as spreading depression.

    Original languageEnglish
    Pages (from-to)323-342
    Number of pages20
    JournalJournal of Basic and Clinical Physiology and Pharmacology
    Volume3
    Issue number4
    DOIs
    StatePublished - Oct 1992

    Bibliographical note

    Funding Information:
    This work was supported in part by NIH Grant NS-22881 and in part by the "Health Science Research Fund, Yad Hanadiv and the Charles Krown Research Fund", and the research committee at Bar-Ilan University.

    Keywords

    • brain ions homeostasis
    • hemoglobin O2 saturation
    • hypoxia and ischemia
    • laser Doppler flowmetry
    • mitochondrial redox state
    • spreading depression

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