Multiparametric evaluation of brain functions in the mongolian gerbil in vivo

A. Mayevsky; K. Frank; M. Muck; M. Kessler; S. Nioka; B. Chance

doi:10.1515/jbcpp.1992.3.4.323

Multiparametric evaluation of brain functions in the mongolian gerbil in vivo

A. Mayevsky, K. Frank, M. Muck, M. Kessler, S. Nioka, B. Chance

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

We have developed the multiprobe assembly (MPA) by which metabolic, ionic and electrical activities can be monitored from the surface of the brain. In the present study we included optical fibers for the monitoring of intracapillary hemoglobin oxygenation by use of the Erlangen Microlight Guide Spectrophotometer (EMPHO-I) from the surface of the gerbil brain. The newly developed MPA provides simultaneous information about oxygen delivery (oxy-deoxy Hb), tissue pC2 level, as well as the intracellular oxygen balance (intramitochondrial redox state). The ionic homeostasis was evaluated by monitoring extracellular K+ and Ca2+ activities reflecting the permeability changes of cation channels as well as the activities of Na+,K+-ATPase and other ion linked transport proces-ses. The electrical activities were monitored by a bipolar electro-cortical surface probe and DC steady potential. The subjects of the present study were Mongolian gerbils (Meriones unguiculatus) anesthetized and operated according to our routine techniques. After 30 min of recovery from the operation each gerbil was exposed to a short anoxia, graded hypoxia, ischemia as well as spreading depression.

Original language	English
Pages (from-to)	323-342
Number of pages	20
Journal	Journal of Basic and Clinical Physiology and Pharmacology
Volume	3
Issue number	4
DOIs	https://doi.org/10.1515/jbcpp.1992.3.4.323
State	Published - Oct 1992

Bibliographical note

Funding Information:
This work was supported in part by NIH Grant NS-22881 and in part by the "Health Science Research Fund, Yad Hanadiv and the Charles Krown Research Fund", and the research committee at Bar-Ilan University.

Funding

This work was supported in part by NIH Grant NS-22881 and in part by the "Health Science Research Fund, Yad Hanadiv and the Charles Krown Research Fund", and the research committee at Bar-Ilan University.

Funders	Funder number
Health Science Research Fund
Yad Hanadiv
National Institutes of Health
National Institute of Neurological Disorders and Stroke	R01NS022881

Keywords

brain ions homeostasis
hemoglobin O2 saturation
hypoxia and ischemia
laser Doppler flowmetry
mitochondrial redox state
spreading depression

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10.1515/jbcpp.1992.3.4.323

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title = "Multiparametric evaluation of brain functions in the mongolian gerbil in vivo",

abstract = "We have developed the multiprobe assembly (MPA) by which metabolic, ionic and electrical activities can be monitored from the surface of the brain. In the present study we included optical fibers for the monitoring of intracapillary hemoglobin oxygenation by use of the Erlangen Microlight Guide Spectrophotometer (EMPHO-I) from the surface of the gerbil brain. The newly developed MPA provides simultaneous information about oxygen delivery (oxy-deoxy Hb), tissue pC2 level, as well as the intracellular oxygen balance (intramitochondrial redox state). The ionic homeostasis was evaluated by monitoring extracellular K+ and Ca2+ activities reflecting the permeability changes of cation channels as well as the activities of Na+,K+-ATPase and other ion linked transport proces-ses. The electrical activities were monitored by a bipolar electro-cortical surface probe and DC steady potential. The subjects of the present study were Mongolian gerbils (Meriones unguiculatus) anesthetized and operated according to our routine techniques. After 30 min of recovery from the operation each gerbil was exposed to a short anoxia, graded hypoxia, ischemia as well as spreading depression.",

keywords = "brain ions homeostasis, hemoglobin O2 saturation, hypoxia and ischemia, laser Doppler flowmetry, mitochondrial redox state, spreading depression",

author = "A. Mayevsky and K. Frank and M. Muck and M. Kessler and S. Nioka and B. Chance",

note = "Funding Information: This work was supported in part by NIH Grant NS-22881 and in part by the {"}Health Science Research Fund, Yad Hanadiv and the Charles Krown Research Fund{"}, and the research committee at Bar-Ilan University.",

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doi = "10.1515/jbcpp.1992.3.4.323",

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AU - Nioka, S.

AU - Chance, B.

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N2 - We have developed the multiprobe assembly (MPA) by which metabolic, ionic and electrical activities can be monitored from the surface of the brain. In the present study we included optical fibers for the monitoring of intracapillary hemoglobin oxygenation by use of the Erlangen Microlight Guide Spectrophotometer (EMPHO-I) from the surface of the gerbil brain. The newly developed MPA provides simultaneous information about oxygen delivery (oxy-deoxy Hb), tissue pC2 level, as well as the intracellular oxygen balance (intramitochondrial redox state). The ionic homeostasis was evaluated by monitoring extracellular K+ and Ca2+ activities reflecting the permeability changes of cation channels as well as the activities of Na+,K+-ATPase and other ion linked transport proces-ses. The electrical activities were monitored by a bipolar electro-cortical surface probe and DC steady potential. The subjects of the present study were Mongolian gerbils (Meriones unguiculatus) anesthetized and operated according to our routine techniques. After 30 min of recovery from the operation each gerbil was exposed to a short anoxia, graded hypoxia, ischemia as well as spreading depression.

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Multiparametric evaluation of brain functions in the mongolian gerbil in vivo

Abstract

Bibliographical note

Funding

Keywords

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