Multiparameter Monitoring and Analysis of In Vivo Ischemic and Hypoxic Heart

Mary Osbakken; Avraham Mayevsky; Mary Osbakken

doi:10.1515/JBCPP.1996.7.2.97

Multiparameter Monitoring and Analysis of In Vivo Ischemic and Hypoxic Heart

Mary Osbakken, Avraham Mayevsky, Mary Osbakken

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We describe a unique in vivo technique which addresses the multifactorial function of the heart, i.e., simultaneous measurement of myocardial ion transport (two mini-electrode systems to measure K⁺_cand Ca²⁺_c), energy metabolism (NADH fluorescence to measure NADH redox state), and coronary flow (laser-Doppler perfusion) using a multiprobe assembly (MPA) which contains transducers for all measurements. The MPA (which is 6 mm in diameter) was applied to the external surface of the heart in an open chest dog model. To test MPA function, myocardial ischemia was produced by application of a balloon occluder to the left anterior descending coronary (LAD) artery, and hypoxia was produced by changing the inspired O₂-N₂ratio until the P_ao₂was 20-30 torr. The MPA simultaneously monitored changes in ion flux, heart metabolism, and tissue perfusion during pathophysiological intervention.

Original language	English
Pages (from-to)	97-114
Number of pages	18
Journal	Journal of Basic and Clinical Physiology and Pharmacology
Volume	7
Issue number	2
DOIs	https://doi.org/10.1515/JBCPP.1996.7.2.97
State	Published - Mar 1996
Externally published	Yes

Keywords

NADH
coronary flow
hypoxia
ion flux
ischemia
redox state
tissue perfusion

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10.1515/JBCPP.1996.7.2.97

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abstract = "We describe a unique in vivo technique which addresses the multifactorial function of the heart, i.e., simultaneous measurement of myocardial ion transport (two mini-electrode systems to measure K+cand Ca2+c), energy metabolism (NADH fluorescence to measure NADH redox state), and coronary flow (laser-Doppler perfusion) using a multiprobe assembly (MPA) which contains transducers for all measurements. The MPA (which is 6 mm in diameter) was applied to the external surface of the heart in an open chest dog model. To test MPA function, myocardial ischemia was produced by application of a balloon occluder to the left anterior descending coronary (LAD) artery, and hypoxia was produced by changing the inspired O2-N2ratio until the Pao2was 20-30 torr. The MPA simultaneously monitored changes in ion flux, heart metabolism, and tissue perfusion during pathophysiological intervention.",

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AB - We describe a unique in vivo technique which addresses the multifactorial function of the heart, i.e., simultaneous measurement of myocardial ion transport (two mini-electrode systems to measure K+cand Ca2+c), energy metabolism (NADH fluorescence to measure NADH redox state), and coronary flow (laser-Doppler perfusion) using a multiprobe assembly (MPA) which contains transducers for all measurements. The MPA (which is 6 mm in diameter) was applied to the external surface of the heart in an open chest dog model. To test MPA function, myocardial ischemia was produced by application of a balloon occluder to the left anterior descending coronary (LAD) artery, and hypoxia was produced by changing the inspired O2-N2ratio until the Pao2was 20-30 torr. The MPA simultaneously monitored changes in ion flux, heart metabolism, and tissue perfusion during pathophysiological intervention.

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Multiparameter Monitoring and Analysis of In Vivo Ischemic and Hypoxic Heart

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Keywords

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