Multiparameter Monitoring and Analysis of In Vivo Ischemic and Hypoxic Heart

Mary Osbakken, Avraham Mayevsky, Mary Osbakken

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


We describe a unique in vivo technique which addresses the multifactorial function of the heart, i.e., simultaneous measurement of myocardial ion transport (two mini-electrode systems to measure K+cand Ca2+c), energy metabolism (NADH fluorescence to measure NADH redox state), and coronary flow (laser-Doppler perfusion) using a multiprobe assembly (MPA) which contains transducers for all measurements. The MPA (which is 6 mm in diameter) was applied to the external surface of the heart in an open chest dog model. To test MPA function, myocardial ischemia was produced by application of a balloon occluder to the left anterior descending coronary (LAD) artery, and hypoxia was produced by changing the inspired O2-N2ratio until the Pao2was 20-30 torr. The MPA simultaneously monitored changes in ion flux, heart metabolism, and tissue perfusion during pathophysiological intervention.

Original languageEnglish
Pages (from-to)97-114
Number of pages18
JournalJournal of Basic and Clinical Physiology and Pharmacology
Issue number2
StatePublished - Mar 1996
Externally publishedYes


  • NADH
  • coronary flow
  • hypoxia
  • ion flux
  • ischemia
  • redox state
  • tissue perfusion


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