TY - GEN
T1 - Multicolor FISH using a novel spectral bioimaging system
AU - Soenksen, Dirk G.
AU - Garini, Yuval
AU - Bar-Am, Irit
PY - 1996
Y1 - 1996
N2 - FISH (fluorescence in situ hybridization) is a cytogenetic technique for locating and quantifying small genetic defects that cannot be detected by traditional karyotyping, the banding analysis of stained chromosomes. The selectivity of FISH holds the promise for the accurate, low cost and fast diagnosis of genetic disorders, birth defects, and various types of cancer that are not detectable by other means. To aid investigators with their FISH measurements, filter-based digital imaging systems are available to enhance and analyze faint FISH images. The accuracy and reliability of traditional FISH measurements is generally compromised when there is (1) sample autofluorescence, (2) image movement, e.g., due to lack of registration between images acquired through different filter sets, or (3) spectral overlap between fluorescent DNA probe emission spectra. SpectraCube TM is an interferometric imaging method which is not subject to the limitations of filters-based systems. By measuring a definitive spectrum, simultaneously at all points in a sample, SpectraCube TM has the potential to revolutionize FISH by enabling the detection and separation of a large number of spectrally and spatially overlapping fluorescent DNA probes, and by eliminating sample autofluorescence. Details of the Applied Spectral Imaging SD200 spectral bio-imaging system and the results of a six-color FISH measurement will be discussed.
AB - FISH (fluorescence in situ hybridization) is a cytogenetic technique for locating and quantifying small genetic defects that cannot be detected by traditional karyotyping, the banding analysis of stained chromosomes. The selectivity of FISH holds the promise for the accurate, low cost and fast diagnosis of genetic disorders, birth defects, and various types of cancer that are not detectable by other means. To aid investigators with their FISH measurements, filter-based digital imaging systems are available to enhance and analyze faint FISH images. The accuracy and reliability of traditional FISH measurements is generally compromised when there is (1) sample autofluorescence, (2) image movement, e.g., due to lack of registration between images acquired through different filter sets, or (3) spectral overlap between fluorescent DNA probe emission spectra. SpectraCube TM is an interferometric imaging method which is not subject to the limitations of filters-based systems. By measuring a definitive spectrum, simultaneously at all points in a sample, SpectraCube TM has the potential to revolutionize FISH by enabling the detection and separation of a large number of spectrally and spatially overlapping fluorescent DNA probes, and by eliminating sample autofluorescence. Details of the Applied Spectral Imaging SD200 spectral bio-imaging system and the results of a six-color FISH measurement will be discussed.
UR - http://www.scopus.com/inward/record.url?scp=0029749706&partnerID=8YFLogxK
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AN - SCOPUS:0029749706
SN - 0819420522
SN - 9780819420527
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 303
EP - 309
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Farkas, Daniel L.
A2 - Leif, Robert C.
A2 - Priezzhev, Alexander V.
A2 - Asakura, Toshimitsu
A2 - Tromberg, Bruce J.
T2 - Optical Diagnostics of Living Cells and Biofluids
Y2 - 28 January 1996 through 1 February 1996
ER -