Multi-spectral imaging in mammalian cells using a home-built LSFM system

K. S. Aiswarya; S. R. Rinsa; Rahul Sharma; Srinivasa Murty Srinivasula; Mayanglambam Suheshkumar Singh

doi:10.1117/12.2649890

Multi-spectral imaging in mammalian cells using a home-built LSFM system

K. S. Aiswarya, S. R. Rinsa, Rahul Sharma, Srinivasa Murty Srinivasula, Mayanglambam Suheshkumar Singh

Indian Institute of Science Education and Research Thiruvananthapuram

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

Light sheet fluorescence microscopy (LSFM) works on fluorescence imaging, employing target-specific fluorescent proteins to label regions of interest in a sample. In this way, analyzing and manipulating information from different targets in a specimen requires labeling the specimen using multiple fluorophores. Multispectral imaging helps identify several target molecules simultaneously at once, thereby retrieving information from the entire volume of the sample. Here we report multispectral imaging studies on mammalian cells using our in-house built multispectral LSFM imaging system. Experimental studies were conducted on mammalian cells in alive conditions, and results are provided.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI
Editors	Attila Tarnok, Jessica P. Houston
Publisher	SPIE
ISBN (Electronic)	9781510658714
DOIs	https://doi.org/10.1117/12.2649890
State	Published - 2023
Externally published	Yes
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023 - San Francisco, United States Duration: 30 Jan 2023 → 1 Feb 2023

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12383
ISSN (Print)	1605-7422

Conference

Conference	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023
Country/Territory	United States
City	San Francisco
Period	30/01/23 → 1/02/23

Bibliographical note

Publisher Copyright:
© 2023 SPIE.

Keywords

HeLa kyoto cells
LSFM
fluorescence imaging
light sheet microscopy
mammalian cells
multispectral imaging

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10.1117/12.2649890

Cite this

Aiswarya, K. S., Rinsa, S. R., Sharma, R., Srinivasula, S. M., & Singh, M. S. (2023). Multi-spectral imaging in mammalian cells using a home-built LSFM system. In A. Tarnok, & J. P. Houston (Eds.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI Article 123830L (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12383). SPIE. https://doi.org/10.1117/12.2649890

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abstract = "Light sheet fluorescence microscopy (LSFM) works on fluorescence imaging, employing target-specific fluorescent proteins to label regions of interest in a sample. In this way, analyzing and manipulating information from different targets in a specimen requires labeling the specimen using multiple fluorophores. Multispectral imaging helps identify several target molecules simultaneously at once, thereby retrieving information from the entire volume of the sample. Here we report multispectral imaging studies on mammalian cells using our in-house built multispectral LSFM imaging system. Experimental studies were conducted on mammalian cells in alive conditions, and results are provided.",

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doi = "10.1117/12.2649890",

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Aiswarya, KS, Rinsa, SR, Sharma, R, Srinivasula, SM & Singh, MS 2023, Multi-spectral imaging in mammalian cells using a home-built LSFM system. in A Tarnok & JP Houston (eds), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI., 123830L, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12383, SPIE, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023, San Francisco, United States, 30/01/23. https://doi.org/10.1117/12.2649890

Multi-spectral imaging in mammalian cells using a home-built LSFM system. / Aiswarya, K. S.; Rinsa, S. R.; Sharma, Rahul et al.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI. ed. / Attila Tarnok; Jessica P. Houston. SPIE, 2023. 123830L (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12383).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Multi-spectral imaging in mammalian cells using a home-built LSFM system

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Keywords

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