Multi-harmonic structured illumination-based optical diffraction tomography

Ruihua Liu; Kai Wen; Jiaoyue Li; Ying Ma; Juanjuan Zheng; Sha An; Junwei Min; Zeev Zalevsky; Baoli Yao; Peng Gao

doi:10.1364/AO.508138

Multi-harmonic structured illumination-based optical diffraction tomography

Ruihua Liu, Kai Wen, Jiaoyue Li, Ying Ma, Juanjuan Zheng, Sha An, Junwei Min, Zeev Zalevsky, Baoli Yao, Peng Gao

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Imaging speed and spatial resolution are key factors in optical diffraction tomography (ODT), while they are mutually exclusive in 3D refractive index imaging. This paper presents a multi-harmonic structured illuminationbased optical diffraction tomography (MHSI-ODT) to acquire 3D refractive index (RI) maps of transparent samples. MHSI-ODT utilizes a digital micromirror device (DMD) to generate structured illumination containing multiple harmonics. For each structured illumination orientation, four spherical spectral crowns are solved from five phase-shifted holograms, meaning that the acquisition of each spectral crown costs 1.25 raw images. Compared to conventional SI-ODT, which retrieves two spectral crowns from three phase-shifted raw images, MHSI-ODT enhances the imaging speed by 16.7% in 3D RI imaging.Meanwhile, MHSI-ODT exploits both the 1st-order and the 2nd-order harmonics; therefore, it has a better intensity utilization of structured illumination. We demonstrated the performance of MHSI-ODT by rendering the 3D RI distributions of 5 mpolystyrene (PS) microspheres and biological samples.

Original language	English
Pages (from-to)	9199-9206
Number of pages	8
Journal	Applied Optics
Volume	62
Issue number	35
DOIs	https://doi.org/10.1364/AO.508138
State	Published - 10 Dec 2023

Bibliographical note

Publisher Copyright:
©2023 Optica Publishing Group.

Funding

Funding. National Key Research and Development Program of China (2022YFE0100700); National Natural Science Foundation of China (62335018, 62075177, 62105251, 12104354); 111 Project; Natural Science Basic Research Program of Shaanxi Province (2022JQ-788); Natural Science Foundation of Shaanxi Province (2023JCQN0731, 2023JCYB518); Fundamental Research Funds for the Central Universities (QTZX23008, QTZX23013, QTZX23024, XJSJ23137).

Funders	Funder number
National Natural Science Foundation of China	62075177, 12104354, 62105251, 62335018
Natural Science Foundation of Shaanxi Province	2023JCQN0731, 2023JCYB518
National Key Research and Development Program of China	2022YFE0100700
Fundamental Research Funds for the Central Universities	XJSJ23137, QTZX23008, QTZX23013, QTZX23024
Higher Education Discipline Innovation Project
Natural Science Basic Research Program of Shaanxi Province	2022JQ-788

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title = "Multi-harmonic structured illumination-based optical diffraction tomography",

abstract = "Imaging speed and spatial resolution are key factors in optical diffraction tomography (ODT), while they are mutually exclusive in 3D refractive index imaging. This paper presents a multi-harmonic structured illuminationbased optical diffraction tomography (MHSI-ODT) to acquire 3D refractive index (RI) maps of transparent samples. MHSI-ODT utilizes a digital micromirror device (DMD) to generate structured illumination containing multiple harmonics. For each structured illumination orientation, four spherical spectral crowns are solved from five phase-shifted holograms, meaning that the acquisition of each spectral crown costs 1.25 raw images. Compared to conventional SI-ODT, which retrieves two spectral crowns from three phase-shifted raw images, MHSI-ODT enhances the imaging speed by 16.7\% in 3D RI imaging.Meanwhile, MHSI-ODT exploits both the 1st-order and the 2nd-order harmonics; therefore, it has a better intensity utilization of structured illumination. We demonstrated the performance of MHSI-ODT by rendering the 3D RI distributions of 5 mpolystyrene (PS) microspheres and biological samples.",

author = "Ruihua Liu and Kai Wen and Jiaoyue Li and Ying Ma and Juanjuan Zheng and Sha An and Junwei Min and Zeev Zalevsky and Baoli Yao and Peng Gao",

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doi = "10.1364/AO.508138",

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AU - Liu, Ruihua

AU - Wen, Kai

AU - Li, Jiaoyue

AU - Ma, Ying

AU - Zheng, Juanjuan

AU - An, Sha

AU - Min, Junwei

AU - Zalevsky, Zeev

AU - Yao, Baoli

AU - Gao, Peng

PY - 2023/12/10

Y1 - 2023/12/10

N2 - Imaging speed and spatial resolution are key factors in optical diffraction tomography (ODT), while they are mutually exclusive in 3D refractive index imaging. This paper presents a multi-harmonic structured illuminationbased optical diffraction tomography (MHSI-ODT) to acquire 3D refractive index (RI) maps of transparent samples. MHSI-ODT utilizes a digital micromirror device (DMD) to generate structured illumination containing multiple harmonics. For each structured illumination orientation, four spherical spectral crowns are solved from five phase-shifted holograms, meaning that the acquisition of each spectral crown costs 1.25 raw images. Compared to conventional SI-ODT, which retrieves two spectral crowns from three phase-shifted raw images, MHSI-ODT enhances the imaging speed by 16.7% in 3D RI imaging.Meanwhile, MHSI-ODT exploits both the 1st-order and the 2nd-order harmonics; therefore, it has a better intensity utilization of structured illumination. We demonstrated the performance of MHSI-ODT by rendering the 3D RI distributions of 5 mpolystyrene (PS) microspheres and biological samples.

AB - Imaging speed and spatial resolution are key factors in optical diffraction tomography (ODT), while they are mutually exclusive in 3D refractive index imaging. This paper presents a multi-harmonic structured illuminationbased optical diffraction tomography (MHSI-ODT) to acquire 3D refractive index (RI) maps of transparent samples. MHSI-ODT utilizes a digital micromirror device (DMD) to generate structured illumination containing multiple harmonics. For each structured illumination orientation, four spherical spectral crowns are solved from five phase-shifted holograms, meaning that the acquisition of each spectral crown costs 1.25 raw images. Compared to conventional SI-ODT, which retrieves two spectral crowns from three phase-shifted raw images, MHSI-ODT enhances the imaging speed by 16.7% in 3D RI imaging.Meanwhile, MHSI-ODT exploits both the 1st-order and the 2nd-order harmonics; therefore, it has a better intensity utilization of structured illumination. We demonstrated the performance of MHSI-ODT by rendering the 3D RI distributions of 5 mpolystyrene (PS) microspheres and biological samples.

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Multi-harmonic structured illumination-based optical diffraction tomography

Abstract

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