Abstract
Super-resolution optical fluctuation imaging (SOFI) offers a simple and affordable alternative to other super-resolution (SR) imaging techniques. The theoretical resolution enhancement of SOFI scales linearly with the order of cumulants, while the imaging conditions exhibit less photo-toxicity to the living samples as compared to other SR methods. High order SOFI could, therefore, be a method of choice for dynamic live cell imaging. However, due to the cusp-artifacts and dynamic range expansion of pixel intensities, this promise has not been materialized as of yet. Here we investigated and compared high order moments vs. high order cumulant SOFI reconstructions. We demonstrate that even-order moments reconstructions are intrinsically free of cusp artifacts, allowing for a subsequent deconvolution operation to be performed, hence enhancing the resolution even further. High order moments reconstruction performance was examined for various (simulated) conditions and applied to (experimental) imaging of QD labeled microtubules in fixed cells, and actin stress fiber dynamics in live cells.
| Original language | English |
|---|---|
| Article number | #353368 |
| Pages (from-to) | 2430-2445 |
| Number of pages | 16 |
| Journal | Biomedical Optics Express |
| Volume | 10 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1 May 2019 |
Bibliographical note
Publisher Copyright:© 2019, OSA - The Optical Society. All rights reserved.
Funding
National Science Foundation (NSF) (1548924); Dean Willard Chair fund; Raymond and Dorothy Wilson Summer Research Fellowship; Japan Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Scientific Research (MEXT KAKENHI) (15H05948); Japan Brain Mapping by Integrated Neurotechnologies for Disease (Brain/MINDS) (14525328); Japan Agency for Medical Research and Development-Core Research for Evolutional Science and Technology (AMED-CREST) (15664816).
| Funders | Funder number |
|---|---|
| Japan Agency for Medical Research and Development-Core Research for Evolutional Science and Technology | |
| Japan Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Scientific Research | |
| National Science Foundation | 1548924 |
| Ministry of Education, Culture, Sports, Science and Technology | 14525328, 15H05948 |
| Core Research for Evolutional Science and Technology | 15664816 |