Molecularly imprinted sensor based on o-aminophenol for the selective determination of norepinephrine in pharmaceutical and biological samples

Rosy, Himanshu Chasta, Rajendra N. Goyal

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


An electrochemical sensor has been developed for the selective determination of norepinephrine (NE) using the molecularly imprinted technique. The imprinted polymer film at the surface of glassy carbon electrode is prepared by the electropolymerization of o-aminophenol in the presence of NE. Imprinted polymer film was characterized by atomic force microscopy (AFM), field emission scanning electron microscopy (FE-SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The imprinted sensor showed a well-defined anodic peak at a potential of ∼ 198 mV in phosphate buffer of pH 7.2 using square wave voltammetry. A linear increase in peak current was found with the increasing concentration of NE in the range from 50×10-9 to 10×10-6 mol L-1and the limit of detection (3σ/b) was found to be 4.9×10-10 mol L-1. The imprinted sensor has been successfully employed to ascertain the content of NE in the commercially available pharmaceutical preparations. The biological applicability of the developed sensor has been delineated by the determination of NE in human plasma and urine samples using the standard addition method. The proposed sensor exhibited high degree of selectivity for NE in comparison to other structurally similar biomolecules present in biological samples, along with long term stability, good reproducibility and excellent capacity of regeneration of molecular recognition sites.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
StatePublished - 1 Jul 2014
Externally publishedYes

Bibliographical note

Funding Information:
Authors (HC and Rosy) are thankful to the Council of Scientific and Industrial Research, New Delhi, and Ministry of Human Resource Development, New Delhi, for the award of Senior Research Fellowship and Junior Research Fellowship, respectively. The financial assistance for this work was provided by the Department of Biotechnology, Ministry of Science and Technology , New Delhi vide Grant no. BT/PR13954/MED/32/143/2010 .


  • Human plasma
  • Human urine
  • Molecularly imprinted technique
  • Norepinephrine
  • Poly(o-aminophenol)


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