Molecular mass distribution of polycations and dextrans by high-performance size exclusion chromatography

Neeraj Kumar; Tony Azzam; Abraham J. Domb

doi:10.1002/pat.245

Molecular mass distribution of polycations and dextrans by high-performance size exclusion chromatography

Neeraj Kumar, Tony Azzam, Abraham J. Domb

Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A high-performance size exclusion chromatographic (HPSEC) method for molecular mass distribution (MMD) was developed for polycations. Results obtained from HPSEC using UV and RI detectors were compared with the viscosity measurements. Pullulan standards were used for M_w calibration. It is concluded that HPSEC with a UV detector offers a sensitive and simple method that has the required accuracy. Degradation studies following this method indicate that dextrans are stable at a temperature of 55°C and at different pH solutions for up to 7 days.

Original language	English
Pages (from-to)	1071-1077
Number of pages	7
Journal	Polymers for Advanced Technologies
Volume	13
Issue number	10-12
DOIs	https://doi.org/10.1002/pat.245
State	Published - 2002
Externally published	Yes

Keywords

Degradation
Dextrans
High-performance size exclusion (HPSEC)
Molecular mass distribution (MMD)
Polycations
Viscosity

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10.1002/pat.245

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abstract = "A high-performance size exclusion chromatographic (HPSEC) method for molecular mass distribution (MMD) was developed for polycations. Results obtained from HPSEC using UV and RI detectors were compared with the viscosity measurements. Pullulan standards were used for Mw calibration. It is concluded that HPSEC with a UV detector offers a sensitive and simple method that has the required accuracy. Degradation studies following this method indicate that dextrans are stable at a temperature of 55°C and at different pH solutions for up to 7 days.",

keywords = "Degradation, Dextrans, High-performance size exclusion (HPSEC), Molecular mass distribution (MMD), Polycations, Viscosity",

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AB - A high-performance size exclusion chromatographic (HPSEC) method for molecular mass distribution (MMD) was developed for polycations. Results obtained from HPSEC using UV and RI detectors were compared with the viscosity measurements. Pullulan standards were used for Mw calibration. It is concluded that HPSEC with a UV detector offers a sensitive and simple method that has the required accuracy. Degradation studies following this method indicate that dextrans are stable at a temperature of 55°C and at different pH solutions for up to 7 days.

KW - Degradation

KW - Dextrans

KW - High-performance size exclusion (HPSEC)

KW - Molecular mass distribution (MMD)

KW - Polycations

KW - Viscosity

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IS - 10-12

ER -

Molecular mass distribution of polycations and dextrans by high-performance size exclusion chromatography

Abstract

Keywords

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