Molecular detection of antibiotic resistance genes from positive blood cultures

Musa Y. Hindiyeh; Gill Smollan; Shiraz Gefen-Halevi; Ella Mendelson; Nathan Keller

doi:10.1007/978-1-4939-1776-1_10

Molecular detection of antibiotic resistance genes from positive blood cultures

Musa Y. Hindiyeh
, Gill Smollan
, Shiraz Gefen-Halevi
, Ella Mendelson
, Nathan Keller

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Rapid detection of the bacterial causative agent causing sepsis must be coupled with rapid identifi cation of the antibiotic resistant mechanism that the pathogen might possess. Real-time PCR (qPCR)-based assays have been extensively utilized in the clinical microbiology fi eld as diagnostic tools for the rapid detection of specifi c nucleic acid (NA) targets. In this chapter, we will discuss the technical aspects of using an internally controlled qPCR assay for the rapid detection of Klebsiella pneumoniae carbapenemase gene (bla_KPC) in positive Bactec blood culture bottles. The multiplex qPCR (bla_KPC /RNase P) utilizes specifi c primers and probes for the detection of the bacterial carbapenem resistance mechanism, bla_KPC gene, and the internal control RNase P. The internal control of the qPCR assay is vital for detecting any inhibitors that are well known to be present in the blood culture bottles. Rapid detection of the antibiotic resistant mechanism present in the bacterial pathogen causing sepsis can help in better managing patients’ infection.

Original language	English
Pages (from-to)	97-108
Number of pages	12
Journal	Methods in Molecular Biology
Volume	1237
DOIs	https://doi.org/10.1007/978-1-4939-1776-1_10
State	Published - 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

Blood culture bottles
Carbapenem resistance
Multidrug resistance
bla KPC

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10.1007/978-1-4939-1776-1_10

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title = "Molecular detection of antibiotic resistance genes from positive blood cultures",

abstract = "Rapid detection of the bacterial causative agent causing sepsis must be coupled with rapid identifi cation of the antibiotic resistant mechanism that the pathogen might possess. Real-time PCR (qPCR)-based assays have been extensively utilized in the clinical microbiology fi eld as diagnostic tools for the rapid detection of specifi c nucleic acid (NA) targets. In this chapter, we will discuss the technical aspects of using an internally controlled qPCR assay for the rapid detection of Klebsiella pneumoniae carbapenemase gene (blaKPC) in positive Bactec blood culture bottles. The multiplex qPCR (blaKPC /RNase P) utilizes specifi c primers and probes for the detection of the bacterial carbapenem resistance mechanism, blaKPC gene, and the internal control RNase P. The internal control of the qPCR assay is vital for detecting any inhibitors that are well known to be present in the blood culture bottles. Rapid detection of the antibiotic resistant mechanism present in the bacterial pathogen causing sepsis can help in better managing patients{\textquoteright} infection.",

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doi = "10.1007/978-1-4939-1776-1\_10",

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AU - Smollan, Gill

AU - Gefen-Halevi, Shiraz

AU - Mendelson, Ella

AU - Keller, Nathan

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - Rapid detection of the bacterial causative agent causing sepsis must be coupled with rapid identifi cation of the antibiotic resistant mechanism that the pathogen might possess. Real-time PCR (qPCR)-based assays have been extensively utilized in the clinical microbiology fi eld as diagnostic tools for the rapid detection of specifi c nucleic acid (NA) targets. In this chapter, we will discuss the technical aspects of using an internally controlled qPCR assay for the rapid detection of Klebsiella pneumoniae carbapenemase gene (blaKPC) in positive Bactec blood culture bottles. The multiplex qPCR (blaKPC /RNase P) utilizes specifi c primers and probes for the detection of the bacterial carbapenem resistance mechanism, blaKPC gene, and the internal control RNase P. The internal control of the qPCR assay is vital for detecting any inhibitors that are well known to be present in the blood culture bottles. Rapid detection of the antibiotic resistant mechanism present in the bacterial pathogen causing sepsis can help in better managing patients’ infection.

AB - Rapid detection of the bacterial causative agent causing sepsis must be coupled with rapid identifi cation of the antibiotic resistant mechanism that the pathogen might possess. Real-time PCR (qPCR)-based assays have been extensively utilized in the clinical microbiology fi eld as diagnostic tools for the rapid detection of specifi c nucleic acid (NA) targets. In this chapter, we will discuss the technical aspects of using an internally controlled qPCR assay for the rapid detection of Klebsiella pneumoniae carbapenemase gene (blaKPC) in positive Bactec blood culture bottles. The multiplex qPCR (blaKPC /RNase P) utilizes specifi c primers and probes for the detection of the bacterial carbapenem resistance mechanism, blaKPC gene, and the internal control RNase P. The internal control of the qPCR assay is vital for detecting any inhibitors that are well known to be present in the blood culture bottles. Rapid detection of the antibiotic resistant mechanism present in the bacterial pathogen causing sepsis can help in better managing patients’ infection.

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ER -

Molecular detection of antibiotic resistance genes from positive blood cultures

Abstract

Bibliographical note

Keywords

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