Molecular characterization and ornithine-urea cycle genes expression in air-breathing magur catfish (Clarias magur) during exposure to high external ammonia

Bodhisattwa Banerjee; Debaprasad Koner; Rubaiya Hasan; Nirmalendu Saha

doi:10.1016/j.ygeno.2019.12.021

Molecular characterization and ornithine-urea cycle genes expression in air-breathing magur catfish (Clarias magur) during exposure to high external ammonia

Bodhisattwa Banerjee
, Debaprasad Koner
, Rubaiya Hasan
, Nirmalendu Saha

Bar-Ilan University - Azrieli Faculty of Medicine

North-Eastern Hill University India

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The air-breathing magur catfish (Clarias magur) is a potential ureogenic teleost because of its functional ornithine-urea cycle (OUC), unlike typical freshwater teleosts. The ability to convert ammonia waste to urea was a significant step towards land-based life forms from aquatic predecessors. Here we investigated the molecular characterization of some OUC genes and the molecular basis of stimulation of ureogenesis via the OUC in magur catfish. The deduced amino acid sequences from the complete cDNA coding sequences of ornithine transcarbamyolase, argininosuccinate synthase, and argininosuccinate lyase indicated that phylogenetically magur catfish is very close to other ureogenic catfishes. Ammonia exposure led to a significant induction of major OUC genes and the gene products in hepatic and in certain non-hepatic tissues of magur catfish. Hence, it is reasonable to assume that the induction of ureogenesis in magur catfish under hyper-ammonia stress is mediated through the activation of OUC genes as an adaptational strategy.

Original language	English
Pages (from-to)	2247-2260
Number of pages	14
Journal	Genomics
Volume	112
Issue number	3
DOIs	https://doi.org/10.1016/j.ygeno.2019.12.021
State	Published - May 2020

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Inc.

Funding

This study was supported by a project sanctioned to NS by the Science and Engineering Research Board ( SR/SO/AS-88/2013 ), New Delhi, the National Agricultural Science Fund ( ABA-7011/1018-19/240 ), New Delhi, and the Department of Biotechnology , New Delhi. This paper is dedicated to Prof. B. K. Ratha on his 72nd Birthday. Appendix A This study was supported by a project sanctioned to NS by the Science and Engineering Research Board (SR/SO/AS-88/2013), New Delhi, the National Agricultural Science Fund (ABA-7011/1018-19/240), New Delhi, and the Department of Biotechnology, New Delhi. This paper is dedicated to Prof. B. K. Ratha on his 72nd Birthday.

Funders	Funder number
Department of Biotechnology , New Delhi
National Agricultural Science Fund
National Academy of Agricultural Sciences	ABA-7011/1018-19/240
Science and Engineering Research Board	SR/SO/AS-88/2013
Department of Biotechnology, Government of West Bengal

Keywords

Gene induction
Hyper-ammonia stress
Molecular phylogenetics
Protein expression
Sequence analysis
Ureogenesis

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10.1016/j.ygeno.2019.12.021

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abstract = "The air-breathing magur catfish (Clarias magur) is a potential ureogenic teleost because of its functional ornithine-urea cycle (OUC), unlike typical freshwater teleosts. The ability to convert ammonia waste to urea was a significant step towards land-based life forms from aquatic predecessors. Here we investigated the molecular characterization of some OUC genes and the molecular basis of stimulation of ureogenesis via the OUC in magur catfish. The deduced amino acid sequences from the complete cDNA coding sequences of ornithine transcarbamyolase, argininosuccinate synthase, and argininosuccinate lyase indicated that phylogenetically magur catfish is very close to other ureogenic catfishes. Ammonia exposure led to a significant induction of major OUC genes and the gene products in hepatic and in certain non-hepatic tissues of magur catfish. Hence, it is reasonable to assume that the induction of ureogenesis in magur catfish under hyper-ammonia stress is mediated through the activation of OUC genes as an adaptational strategy.",

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Molecular characterization and ornithine-urea cycle genes expression in air-breathing magur catfish (Clarias magur) during exposure to high external ammonia

Abstract

Bibliographical note

Funding

Keywords

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