Modified PVA-Fe3O4 nanopartides as protein carriers into sperm cells

Shirly Ben David Makhluf, Riam Abu-Mukh, Sara Rubinstein, Haim Breitbart, Aharon Gedanken

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Magnetite nanopartides conjugated to protein are developed in order to potentially serve as protein carriers into bovine sperm cells. The conjugate comprises iron oxide nanoparticles that are covalently bound to an antiprotein kinase C (PKC)α antibody. This conjugate can serve for cellular PKC localization and the inhibition of its function. The surface of the nanoparticle is first modified with (3-aminopropyl) thrimethoxysilane to form a self-assembled monolayer, and subsequently conjugated with the antibody through amidation between the carboxylic acid end groups on the antibody and the amine groups on the surface of the nanopartides. The anti-PKCα localization is proven by fluorescent microscopy and iron staining. The activity of the anti-PKCα conjugated with the nanoparticle is tested by recognizing PKCα using the Western blot method.

Original languageEnglish
Pages (from-to)1453-1458
Number of pages6
JournalSmall
Volume4
Issue number9
DOIs
StatePublished - Sep 2008

Keywords

  • Anti-PKCα
  • Bovine sperm cells
  • Magnetite nanopartides

Fingerprint

Dive into the research topics of 'Modified PVA-Fe3O4 nanopartides as protein carriers into sperm cells'. Together they form a unique fingerprint.

Cite this