Mitochondrial dysfunction: Bench- To- Bedside optical monitoring of tissue vitality

Avraham Mayevsky, Nava Dekel, Levi Oren, Assaf Deutsch, Eliyahu Pewzner

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

In normal cell the mitochondria are the major source of energy for cellular functions. They serve as biosensors for oxidative stress and involved also in termination of cell function by apoptosis. The involvement of mitochondria in pathological states such as neurodegenerative diseases, sepsis, stroke and cancer are well documented. The involvement of mitochondrial respiration and function in cancer development, proliferation and possible therapy were initiated 75 years ago by Otto Warburg. Monitoring of NADH fluorescence in vivo as an intracellular oxygen indicator was established in the 1950-1970 by Britton Chance and collaborators. In the last 20 years we developed and used a multiparametric monitoring system enabling real time assessment of mitochondria NADH, microcirculatory blood flow and volume as well as HbO2 oxygenation. In order to use this technology in clinical practice the commercial developed device - the "CritiView" was tested in animal models as well as in patients hospitalized in the critical care departments. In patients we tested the viability of the urethral wall (a less - vital tissue) by a 3 way Foley urinary catheter that contains the optical probe. The catheter was introduced to patients underwent open heart by-pass surgery or abdominal aorta aneurysm (AAA) operations. The monitoring started immediately after the insertion of the catheter to the patient and was stopped when the patient was discharged from the operation room. The results show that monitoring of the vitality of the Urethral wall provides information in correlation to the surgical procedure performed. In the AAA patients the occlusion of the aorta led to severe ischemia developed in the urethral wall and recovery of signals were recorded after the reopening of the aorta. In patients under went heart bypass surgery the urethra vitality was decreased dramatically during the operation and recovery was noted in most patients after the discharge of the patient from the operation room.

Original languageEnglish
Title of host publicationBiomedical Optical Spectroscopy
DOIs
StatePublished - 2008
EventBiomedical Optical Spectroscopy - San Jose, CA, United States
Duration: 19 Jan 200823 Jan 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6853
ISSN (Print)1605-7422

Conference

ConferenceBiomedical Optical Spectroscopy
Country/TerritoryUnited States
CitySan Jose, CA
Period19/01/0823/01/08

Keywords

  • CritiView
  • Hemoglobin oxygenation
  • Microcirculatory blood flow
  • Mitochondrial dysfunction
  • NADH redox state
  • Patient monitoring

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