Microscopic and mesoscopic spectral bioimaging

Daniel L. Farkas, Byron T. Ballou, Gregory W. Fisher, Daniel Fishman, Yuval Garini, Wen hua Niu, Elliot S. Wachman

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

16 Scopus citations


Light microscopy has become a versatile tool for investigating biological phenomena as they unfold, using cells as living microcuvettes. The progress is based on improvements in a number of technological fields, including optics, electronics, reagent chemistry and computer science. The non-destructiveness, spatial resolution and speed of optical imaging can provide high versatility for investigating biological structure and function at the tissue or even whole organism level as well. Having previously reported on some new approaches to meeting the challenges posed by the fluorescence-based imaging of cells and tissues, we concentrate here on imaging with increased spectral content and resolution. We improved and applied two techniques of spectral selection: (1) acousto-optic tunable filtering, allowing for multiwavelength fluorescence microscopy with diffraction-limited spatial imaging and sub- millisecond temporal resolution, and (2) 2D Sagnac interferometry-based Fourier spectroscopy, yielding advanced spectral imaging capabilities. Enhanced implementations of existing optical technologies, coupled with image processing, were key in these approaches. We present an overview of the methods, and summarize some of our results in applying these advances to imaging biological specimens. The extension of spectral selection approaches to the mesoscopic domain, suitable for in vivo imaging is also illustrated, by fluorescence-based tumor visualization in the near infrared spectral region. Finally, some future directions are discussed.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsDaniel L. Farkas, Robert C. Leif, Alexander V. Priezzhev, Toshimitsu Asakura, Bruce J. Tromberg
Number of pages10
StatePublished - 1996
Externally publishedYes
EventOptical Diagnostics of Living Cells and Biofluids - San Jose, CA, USA
Duration: 28 Jan 19961 Feb 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering


ConferenceOptical Diagnostics of Living Cells and Biofluids
CitySan Jose, CA, USA


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